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A sweetpotato gene index established by de novo assembly of pyrosequencing and Sanger sequences and mining for gene-based microsatellite markers

Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing se...

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Main Authors: Schafleitner, R., Tincopa, L.R., Palomino, O., Rossel, G., Robles, R., Alagon, R., Rivera, C., Quispe, C., Rojas, L., Pacheco, J., Solis, J., Cerna, D., Kim, J.Y., Hou, J., Simon, R.
Format: Journal Article
Language:Inglés
Published: Springer 2010
Subjects:
sweet potatoes
genes
nucleotide sequence
genetic markers
Online Access:https://hdl.handle.net/10568/97198
Summary:Sweetpotato (Ipomoea batatas (L.) Lam.), a hexaploid outcrossing crop, is an important staple and food security crop in developing countries in Africa and Asia. The availability of genomic resources for sweetpotato is in striking contrast to its importance for human nutrition. Previously existing sequence data were restricted to around 22,000 expressed sequence tag (EST) sequences and ~ 1,500 GenBank sequences. We have used 454 pyrosequencing to augment the available gene sequence information to enhance functional genomics and marker design for this plant species.

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