Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms
A new galactose-specific lectin was isolated from African yam bean (Sphenostyles stenocarpa Harms) by affinity chromatography on galactose-Sepharose 4B. SDS–PAGE analysis resulted in four polypeptide bands of approximately 27, 29, 32 and 34 kDa, respectively. Based on the analysis of carbohydrate co...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1999
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/96012 |
| _version_ | 1855532778929520640 |
|---|---|
| author | Machuka, J.S. Okeola, O.G. Els, J.V.D. Chrispeels, M.J. Leuven, F. van Peumans, W.J. |
| author_browse | Chrispeels, M.J. Els, J.V.D. Leuven, F. van Machuka, J.S. Okeola, O.G. Peumans, W.J. |
| author_facet | Machuka, J.S. Okeola, O.G. Els, J.V.D. Chrispeels, M.J. Leuven, F. van Peumans, W.J. |
| author_sort | Machuka, J.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A new galactose-specific lectin was isolated from African yam bean (Sphenostyles stenocarpa Harms) by affinity chromatography on galactose-Sepharose 4B. SDS–PAGE analysis resulted in four polypeptide bands of approximately 27, 29, 32 and 34 kDa, respectively. Based on the analysis of carbohydrate content and native PAGE, it is likely that the Sphenostyles lectin is a tetrameric glycoprotein with Mr of approximately 122 kDa. N-terminal protein sequencing of purified lectins from four different Sphenostyles accessions shows that the four polypeptides have largely identical amino acid sequences. The sequences contain the conserved consensus sequence F-F–LILG characteristic of legume lectins, as well as Phaseolus vulgaris proteins in the arcelin-α-amylase inhibitor gene family. The lectin agglutinates both rabbit and human erythrocytes, but with a preference for blood types A and O. Using Western blotting, the lectin was shown to accumulate rapidly during seed development, but levels dropped slightly as seeds attained maturity. This is the first time a lectin has been purified from the genus Sphenostyles. The new lectin was assigned the abbreviation LECp.SphSte.se.Hga1. |
| format | Journal Article |
| id | CGSpace96012 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| record_format | dspace |
| spelling | CGSpace960122023-06-12T18:29:22Z Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms Machuka, J.S. Okeola, O.G. Els, J.V.D. Chrispeels, M.J. Leuven, F. van Peumans, W.J. african yam beans leguminosae lectins purification seed development A new galactose-specific lectin was isolated from African yam bean (Sphenostyles stenocarpa Harms) by affinity chromatography on galactose-Sepharose 4B. SDS–PAGE analysis resulted in four polypeptide bands of approximately 27, 29, 32 and 34 kDa, respectively. Based on the analysis of carbohydrate content and native PAGE, it is likely that the Sphenostyles lectin is a tetrameric glycoprotein with Mr of approximately 122 kDa. N-terminal protein sequencing of purified lectins from four different Sphenostyles accessions shows that the four polypeptides have largely identical amino acid sequences. The sequences contain the conserved consensus sequence F-F–LILG characteristic of legume lectins, as well as Phaseolus vulgaris proteins in the arcelin-α-amylase inhibitor gene family. The lectin agglutinates both rabbit and human erythrocytes, but with a preference for blood types A and O. Using Western blotting, the lectin was shown to accumulate rapidly during seed development, but levels dropped slightly as seeds attained maturity. This is the first time a lectin has been purified from the genus Sphenostyles. The new lectin was assigned the abbreviation LECp.SphSte.se.Hga1. 1999 2018-07-05T06:30:27Z 2018-07-05T06:30:27Z Journal Article https://hdl.handle.net/10568/96012 en Limited Access Machuka, J.S., Okeola, O.G., Els, J.V.D., Chrispeels, M.J., Van Leuven, F. & Peumans, W.J. (1999). Isolation and partial characterisation of galactose-specific lectins from African yam beans, Sphenostyles stenocarpa Harms. Phytochemistry, 51(6), 721-728. |
| spellingShingle | african yam beans leguminosae lectins purification seed development Machuka, J.S. Okeola, O.G. Els, J.V.D. Chrispeels, M.J. Leuven, F. van Peumans, W.J. Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms |
| title | Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms |
| title_full | Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms |
| title_fullStr | Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms |
| title_full_unstemmed | Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms |
| title_short | Isolation and partial characterisation of galactosespecific lectins from African yam beans, Sphenostylis stenocarpa Harms |
| title_sort | isolation and partial characterisation of galactosespecific lectins from african yam beans sphenostylis stenocarpa harms |
| topic | african yam beans leguminosae lectins purification seed development |
| url | https://hdl.handle.net/10568/96012 |
| work_keys_str_mv | AT machukajs isolationandpartialcharacterisationofgalactosespecificlectinsfromafricanyambeanssphenostylisstenocarpaharms AT okeolaog isolationandpartialcharacterisationofgalactosespecificlectinsfromafricanyambeanssphenostylisstenocarpaharms AT elsjvd isolationandpartialcharacterisationofgalactosespecificlectinsfromafricanyambeanssphenostylisstenocarpaharms AT chrispeelsmj isolationandpartialcharacterisationofgalactosespecificlectinsfromafricanyambeanssphenostylisstenocarpaharms AT leuvenfvan isolationandpartialcharacterisationofgalactosespecificlectinsfromafricanyambeanssphenostylisstenocarpaharms AT peumanswj isolationandpartialcharacterisationofgalactosespecificlectinsfromafricanyambeanssphenostylisstenocarpaharms |