Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels
Aflatoxin contamination is associated with the development of aflatoxigenic fungi such as Aspergillus flavus and A. parasiticus on food grains. This study was aimed at investigating metabolites produced during fungal development on maize and their correlation with aflatoxin levels. Maize cobs were h...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2018
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| Online Access: | https://hdl.handle.net/10568/92880 |
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| author | Falade, Titilayo D.O. Chrysanthopoulos, P.K. Hodson, M.P. Sultanbawa, Y. Fletcher, M. Darnell, Ross Korie, S. Fox, G. |
| author_browse | Chrysanthopoulos, P.K. Darnell, Ross Falade, Titilayo D.O. Fletcher, M. Fox, G. Hodson, M.P. Korie, S. Sultanbawa, Y. |
| author_facet | Falade, Titilayo D.O. Chrysanthopoulos, P.K. Hodson, M.P. Sultanbawa, Y. Fletcher, M. Darnell, Ross Korie, S. Fox, G. |
| author_sort | Falade, Titilayo D.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Aflatoxin contamination is associated with the development of aflatoxigenic fungi such as Aspergillus flavus and A. parasiticus on food grains. This study was aimed at investigating metabolites produced during fungal development on maize and their correlation with aflatoxin levels. Maize cobs were harvested at R3 (milk), R4 (dough), and R5 (dent) stages of maturity. Individual kernels were inoculated in petri dishes with four doses of fungal spores. Fungal colonisation, metabolite profile, and aflatoxin levels were examined. Grain colonisation decreased with kernel maturity: milk-, dough-, and dent-stage kernels by approximately 100%, 60%, and 30% respectively. Aflatoxin levels increased with dose at dough and dent stages. Polar metabolites including alanine, proline, serine, valine, inositol, iso-leucine, sucrose, fructose, trehalose, turanose, mannitol, glycerol, arabitol, inositol,
myo-inositol, and some intermediates of the tricarboxylic acid cycle (TCA—also known as citric acid or Krebs cycle) were important for dose classification. Important non-polar metabolites included arachidic, palmitic, stearic, 3,4-xylylic, and margaric acids. Aflatoxin levels correlated with levels of several polar metabolites. The strongest positive and negative correlations were with arabitol (R = 0.48) and turanose and (R = 0.53), respectively. Several metabolites were interconnected with the TCA; interconnections of the metabolites with the TCA cycle varied depending upon the grain maturity. |
| format | Journal Article |
| id | CGSpace92880 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace928802025-11-11T10:15:40Z Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels Falade, Titilayo D.O. Chrysanthopoulos, P.K. Hodson, M.P. Sultanbawa, Y. Fletcher, M. Darnell, Ross Korie, S. Fox, G. aflatoxins aspergillus parasiticus metabolites maize grain s Aflatoxin contamination is associated with the development of aflatoxigenic fungi such as Aspergillus flavus and A. parasiticus on food grains. This study was aimed at investigating metabolites produced during fungal development on maize and their correlation with aflatoxin levels. Maize cobs were harvested at R3 (milk), R4 (dough), and R5 (dent) stages of maturity. Individual kernels were inoculated in petri dishes with four doses of fungal spores. Fungal colonisation, metabolite profile, and aflatoxin levels were examined. Grain colonisation decreased with kernel maturity: milk-, dough-, and dent-stage kernels by approximately 100%, 60%, and 30% respectively. Aflatoxin levels increased with dose at dough and dent stages. Polar metabolites including alanine, proline, serine, valine, inositol, iso-leucine, sucrose, fructose, trehalose, turanose, mannitol, glycerol, arabitol, inositol, myo-inositol, and some intermediates of the tricarboxylic acid cycle (TCA—also known as citric acid or Krebs cycle) were important for dose classification. Important non-polar metabolites included arachidic, palmitic, stearic, 3,4-xylylic, and margaric acids. Aflatoxin levels correlated with levels of several polar metabolites. The strongest positive and negative correlations were with arabitol (R = 0.48) and turanose and (R = 0.53), respectively. Several metabolites were interconnected with the TCA; interconnections of the metabolites with the TCA cycle varied depending upon the grain maturity. 2018 2018-05-24T14:44:53Z 2018-05-24T14:44:53Z Journal Article https://hdl.handle.net/10568/92880 en Open Access application/pdf MDPI Falade, T.D., Chrysanthopoulos, P.K., Hodson, M.P., Sultanbawa, Y., Fletcher, M., Darnell, R., ... & Fox, G. (2018). Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels. Toxins, 10(5), 1-22. |
| spellingShingle | aflatoxins aspergillus parasiticus metabolites maize grain s Falade, Titilayo D.O. Chrysanthopoulos, P.K. Hodson, M.P. Sultanbawa, Y. Fletcher, M. Darnell, Ross Korie, S. Fox, G. Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels |
| title | Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels |
| title_full | Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels |
| title_fullStr | Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels |
| title_full_unstemmed | Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels |
| title_short | Metabolites identified during varied doses of aspergillus species in Zea mays grains, and their correlation with aflatoxin levels |
| title_sort | metabolites identified during varied doses of aspergillus species in zea mays grains and their correlation with aflatoxin levels |
| topic | aflatoxins aspergillus parasiticus metabolites maize grain s |
| url | https://hdl.handle.net/10568/92880 |
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