Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm
Aspergillus flavus induced ear rots and subsequent contamination of maize (Zea mays L.) by aflatoxin is a serious food safety issue, especially in developing countries where the crop is mostly cultivated by smallholder famers for own consumption and income generation. A better understanding of the m...
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| Format: | Journal Article |
| Language: | Inglés |
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Brill
2018
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| Online Access: | https://hdl.handle.net/10568/92550 |
| _version_ | 1855524344358240256 |
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| author | Garrido-Bazan, V. Mahuku, George S. Bibbins-Martinez, M. Arroyo-Bacerra, A. Villalobos-López, M.A. |
| author_browse | Arroyo-Bacerra, A. Bibbins-Martinez, M. Garrido-Bazan, V. Mahuku, George S. Villalobos-López, M.A. |
| author_facet | Garrido-Bazan, V. Mahuku, George S. Bibbins-Martinez, M. Arroyo-Bacerra, A. Villalobos-López, M.A. |
| author_sort | Garrido-Bazan, V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Aspergillus flavus induced ear rots and subsequent contamination of maize (Zea mays L.) by aflatoxin is a serious food safety issue, especially in developing countries where the crop is mostly cultivated by smallholder famers for own consumption and income generation. A better understanding of the mechanisms of resistance could help breeders to develop resistant maize varieties. In this study, a set of six tropical maize inbred lines previously identified as resistant or susceptible under natural field conditions were evaluated for response to A. flavus colonisation and aflatoxin contamination. Fungal biomass was significantly higher (P<0.05) in susceptible than resistant maize inbred lines, and this was highly correlated (P=0.001) to aflatoxin levels. Maize inbred lines MRI, MR2 and MR3 had low fungal biomass and low aflatoxin levels, suggesting that resistance in these lines was mediated through restricted fungal colonisation and establishment. Among the three putatively resistant inbred lines mentioned above, MR2 had a relatively high colonisation compared to the other two lines, revealing that A. flavus could establish and colonise kernels that were injured during inoculation, but did not contain high levels of aflatoxin. This could signify the presence of host genes that interfere with the aflatoxin biosynthetic pathway. |
| format | Journal Article |
| id | CGSpace92550 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Brill |
| publisherStr | Brill |
| record_format | dspace |
| spelling | CGSpace925502025-01-27T15:00:52Z Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm Garrido-Bazan, V. Mahuku, George S. Bibbins-Martinez, M. Arroyo-Bacerra, A. Villalobos-López, M.A. maize aspergillus flavus aflatoxins resistance food security Aspergillus flavus induced ear rots and subsequent contamination of maize (Zea mays L.) by aflatoxin is a serious food safety issue, especially in developing countries where the crop is mostly cultivated by smallholder famers for own consumption and income generation. A better understanding of the mechanisms of resistance could help breeders to develop resistant maize varieties. In this study, a set of six tropical maize inbred lines previously identified as resistant or susceptible under natural field conditions were evaluated for response to A. flavus colonisation and aflatoxin contamination. Fungal biomass was significantly higher (P<0.05) in susceptible than resistant maize inbred lines, and this was highly correlated (P=0.001) to aflatoxin levels. Maize inbred lines MRI, MR2 and MR3 had low fungal biomass and low aflatoxin levels, suggesting that resistance in these lines was mediated through restricted fungal colonisation and establishment. Among the three putatively resistant inbred lines mentioned above, MR2 had a relatively high colonisation compared to the other two lines, revealing that A. flavus could establish and colonise kernels that were injured during inoculation, but did not contain high levels of aflatoxin. This could signify the presence of host genes that interfere with the aflatoxin biosynthetic pathway. 2018-04-25 2018-05-15T09:42:29Z 2018-05-15T09:42:29Z Journal Article https://hdl.handle.net/10568/92550 en Limited Access Brill Garrido-Bazan, V., Mahuku, G., Bibbins-Martinez, M., Arroyo-Bacerra, A. & Villalobos-López, M.Á. (2018). Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm. World Mycotoxin Journal, 11(2), 215-224. |
| spellingShingle | maize aspergillus flavus aflatoxins resistance food security Garrido-Bazan, V. Mahuku, George S. Bibbins-Martinez, M. Arroyo-Bacerra, A. Villalobos-López, M.A. Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm |
| title | Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm |
| title_full | Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm |
| title_fullStr | Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm |
| title_full_unstemmed | Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm |
| title_short | Dissection of mechanisms of resistance to Aspergillus flavus and aflatoxin using tropical maize germplasm |
| title_sort | dissection of mechanisms of resistance to aspergillus flavus and aflatoxin using tropical maize germplasm |
| topic | maize aspergillus flavus aflatoxins resistance food security |
| url | https://hdl.handle.net/10568/92550 |
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