Rapid detection of potyviruses from crude plant extracts
Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particular...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/92398 |
| _version_ | 1855515973008752640 |
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| author | Silva, G. Oyekanmi, J. Nkere, C.K. Bömer, M. Kumar, P. Lava Seal, S.E. |
| author_browse | Bömer, M. Kumar, P. Lava Nkere, C.K. Oyekanmi, J. Seal, S.E. Silva, G. |
| author_facet | Silva, G. Oyekanmi, J. Nkere, C.K. Bömer, M. Kumar, P. Lava Seal, S.E. |
| author_sort | Silva, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of polysaccharides and polyphenols such as yam (Dioscorea spp.). Here, we report the development of a reliable, rapid and cost-effective detection method for the two most important potyviruses infecting yam based on reverse transcription-recombinase polymerase amplification (RT-RPA).
The developed method, named ‘Direct RT-RPA’, detects each target virus directly from plant leaf extracts prepared with a simple and inexpensive extraction method avoiding laborious extraction of high-quality RNA. Direct RT-RPA enables the detection of virus-positive samples in under 30 min at a single low operation temperature (37 °C) without the need for any expensive instrumentation.
The Direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories and seed certification facilities worldwide. |
| format | Journal Article |
| id | CGSpace92398 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace923982025-11-11T10:04:12Z Rapid detection of potyviruses from crude plant extracts Silva, G. Oyekanmi, J. Nkere, C.K. Bömer, M. Kumar, P. Lava Seal, S.E. yams potyvirus plant viruses food security recombinase polymerase amplification isothermal amplification rapid diagnostics crude plant extracts cell biology molecular biology biochemistry biophysics Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of polysaccharides and polyphenols such as yam (Dioscorea spp.). Here, we report the development of a reliable, rapid and cost-effective detection method for the two most important potyviruses infecting yam based on reverse transcription-recombinase polymerase amplification (RT-RPA). The developed method, named ‘Direct RT-RPA’, detects each target virus directly from plant leaf extracts prepared with a simple and inexpensive extraction method avoiding laborious extraction of high-quality RNA. Direct RT-RPA enables the detection of virus-positive samples in under 30 min at a single low operation temperature (37 °C) without the need for any expensive instrumentation. The Direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories and seed certification facilities worldwide. 2018-04 2018-04-26T15:28:51Z 2018-04-26T15:28:51Z Journal Article https://hdl.handle.net/10568/92398 en Open Access application/pdf Elsevier Silva, G., Oyekanmi, J., Nkere, C.K., Bömer, M., Kumar, P.L. & Seal, S.E. (2018). Rapid detection of potyviruses from crude plant extracts. Analytical Biochemistry, 546, 17-22. |
| spellingShingle | yams potyvirus plant viruses food security recombinase polymerase amplification isothermal amplification rapid diagnostics crude plant extracts cell biology molecular biology biochemistry biophysics Silva, G. Oyekanmi, J. Nkere, C.K. Bömer, M. Kumar, P. Lava Seal, S.E. Rapid detection of potyviruses from crude plant extracts |
| title | Rapid detection of potyviruses from crude plant extracts |
| title_full | Rapid detection of potyviruses from crude plant extracts |
| title_fullStr | Rapid detection of potyviruses from crude plant extracts |
| title_full_unstemmed | Rapid detection of potyviruses from crude plant extracts |
| title_short | Rapid detection of potyviruses from crude plant extracts |
| title_sort | rapid detection of potyviruses from crude plant extracts |
| topic | yams potyvirus plant viruses food security recombinase polymerase amplification isothermal amplification rapid diagnostics crude plant extracts cell biology molecular biology biochemistry biophysics |
| url | https://hdl.handle.net/10568/92398 |
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