Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells
A dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars to study anthocyanin biotransformation in the stomach, small intestine and colonic vessels. Colonic Caco-2 cancer cells and non-tumorigenic colonic CCD-112CoN cells were...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2017
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| Online Access: | https://hdl.handle.net/10568/91982 |
| _version_ | 1855533997383221248 |
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| author | Kubow, S. Iskandar, M.M. Melgar-Bermudez, E. Sleno, L. Sabally, K. Azadi, B. How, E. Prakash, S. Burgos, G. Felde, T. zum |
| author_browse | Azadi, B. Burgos, G. Felde, T. zum How, E. Iskandar, M.M. Kubow, S. Melgar-Bermudez, E. Prakash, S. Sabally, K. Sleno, L. |
| author_facet | Kubow, S. Iskandar, M.M. Melgar-Bermudez, E. Sleno, L. Sabally, K. Azadi, B. How, E. Prakash, S. Burgos, G. Felde, T. zum |
| author_sort | Kubow, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars to study anthocyanin biotransformation in the stomach, small intestine and colonic vessels. Colonic Caco-2 cancer cells and non-tumorigenic colonic CCD-112CoN cells were tested for cytotoxicity and cell viability after 24 h exposure to colonic fecal water (FW) digests (0%, 10%, 25%, 75% and 100% FW in culture media). After 24 h digestion, liquid chromatography-mass spectrometry identified 36 and 15 anthocyanin species throughout the GI vessels for Amachi and Leona, respectively. The total anthocyanin concentration was over thirty-fold higher in Amachi compared to Leona digests but seven-fold higher anthocyanin concentrations were noted for Leona versus Amachi in descending colon digests. Leona FW showed greater potency to induce cytotoxicity and decrease viability of Caco-2 cells than observed with FW from Amachi. Amachi FW at 100% caused cytotoxicity in non-tumorigenic cells while FW from Leona showed no effect. The present findings indicate major variations in the pattern of anthocyanin breakdown and release during digestion of purple-fleshed cultivars. The differing microbial anthocyanin metabolite profiles in colonic vessels between cultivars could play a significant role in the impact of FW toxicity on tumor and non-tumorigenic cells. |
| format | Journal Article |
| id | CGSpace91982 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace919822023-12-08T19:36:04Z Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells Kubow, S. Iskandar, M.M. Melgar-Bermudez, E. Sleno, L. Sabally, K. Azadi, B. How, E. Prakash, S. Burgos, G. Felde, T. zum potatoes anthocyanins digestive system antioxidants neoplasms cytotoxicity A dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars to study anthocyanin biotransformation in the stomach, small intestine and colonic vessels. Colonic Caco-2 cancer cells and non-tumorigenic colonic CCD-112CoN cells were tested for cytotoxicity and cell viability after 24 h exposure to colonic fecal water (FW) digests (0%, 10%, 25%, 75% and 100% FW in culture media). After 24 h digestion, liquid chromatography-mass spectrometry identified 36 and 15 anthocyanin species throughout the GI vessels for Amachi and Leona, respectively. The total anthocyanin concentration was over thirty-fold higher in Amachi compared to Leona digests but seven-fold higher anthocyanin concentrations were noted for Leona versus Amachi in descending colon digests. Leona FW showed greater potency to induce cytotoxicity and decrease viability of Caco-2 cells than observed with FW from Amachi. Amachi FW at 100% caused cytotoxicity in non-tumorigenic cells while FW from Leona showed no effect. The present findings indicate major variations in the pattern of anthocyanin breakdown and release during digestion of purple-fleshed cultivars. The differing microbial anthocyanin metabolite profiles in colonic vessels between cultivars could play a significant role in the impact of FW toxicity on tumor and non-tumorigenic cells. 2017-09 2018-04-02T20:39:31Z 2018-04-02T20:39:31Z Journal Article https://hdl.handle.net/10568/91982 en Open Access MDPI Kubow, S,; Iskandar, M.M.; Melgar-Bermudez, E.; Sleno, L.; Sabally, K.; Azadi, B.; How, E.; Prakash, S.; Burgos, G.; Felde, T. zum. 2017. Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells. Nutrients. (Switzerland). ISSN 2072-6643. 9(953). |
| spellingShingle | potatoes anthocyanins digestive system antioxidants neoplasms cytotoxicity Kubow, S. Iskandar, M.M. Melgar-Bermudez, E. Sleno, L. Sabally, K. Azadi, B. How, E. Prakash, S. Burgos, G. Felde, T. zum Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells |
| title | Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells |
| title_full | Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells |
| title_fullStr | Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells |
| title_full_unstemmed | Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells |
| title_short | Effects of simulated human gastrointestinal digestion of two purple-fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non-tumorigenic cells |
| title_sort | effects of simulated human gastrointestinal digestion of two purple fleshed potato cultivars on anthocyanin composition and cytotoxicity in colonic cancer and non tumorigenic cells |
| topic | potatoes anthocyanins digestive system antioxidants neoplasms cytotoxicity |
| url | https://hdl.handle.net/10568/91982 |
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