Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia
igeon pea (Cajanus cajan (L.) Millsp.) is an important protein source grown in several tropical and sub-tropical countries, and is considered a multi-purpose plant that is resistant to the conditions where drought and salinity is a common phenomenon. The aim of this study was to evaluate the diversi...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Academic Journals
2018
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/91713 |
| _version_ | 1855533491717931008 |
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| author | Degefu, T. Woldemeskel, Endalkachew Adem, M. Fikre, Asnake Amede, Tilahun Ojiewo, Christopher Ochieng |
| author_browse | Adem, M. Amede, Tilahun Degefu, T. Fikre, Asnake Ojiewo, Christopher Ochieng Woldemeskel, Endalkachew |
| author_facet | Degefu, T. Woldemeskel, Endalkachew Adem, M. Fikre, Asnake Amede, Tilahun Ojiewo, Christopher Ochieng |
| author_sort | Degefu, T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | igeon pea (Cajanus cajan (L.) Millsp.) is an important protein source grown in several tropical and sub-tropical countries, and is considered a multi-purpose plant that is resistant to the conditions where drought and salinity is a common phenomenon. The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeon pea plants grown in central and southern Ethiopia. A total of 116 nitrogen-fixing rhizobial strains were isolated. The bacterial isolates were characterized by 91 phenotypic traits including cultural characteristics, intrinsic antibiotic and heavy metal resistance, salt, pH and incubation temperature tolerance, and carbon and nitrogen sources utilization ability. Preliminary symbiotic properties of the isolates were also evaluated. The isolates were compared with seven reference strains of rhizobia by application of the unweighted pair group method with arithmetic means (UPGMA) using NTSYSpc Version 2.1 software program. The dendrogram constructed from cluster analysis of 91 phenotypic traits, grouped them into six clusters and eight un-clustered positions at 80% relative similarity. Cluster I contained 83% of the test isolates that were grouped together with the reference strains Bradyrhizobium japonicum (HAMBI 2314T) and Bradyrhizobium elkanii (LMG 6164), suggesting that pigeon pea is commonly nodulated by bradyrhizobia. Results from symbiotic effectiveness test revealed that majority of the isolates were found to be effective. Generally, this investigation demonstrated that rhizobial population nodulating C. cajan on the study area were phenotypically diverse and symbiotically effective. Furthermore, the result indicates the existence of strains in the collection, which can tolerate environmental stresses, thus can be developed into inoculant for pigeon pea inoculation and production in Ethiopia and beyond. |
| format | Journal Article |
| id | CGSpace91713 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Academic Journals |
| publisherStr | Academic Journals |
| record_format | dspace |
| spelling | CGSpace917132024-04-25T06:01:25Z Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia Degefu, T. Woldemeskel, Endalkachew Adem, M. Fikre, Asnake Amede, Tilahun Ojiewo, Christopher Ochieng plants pigeon peas research bradyrhizobium cajanus cajan rhizobium igeon pea (Cajanus cajan (L.) Millsp.) is an important protein source grown in several tropical and sub-tropical countries, and is considered a multi-purpose plant that is resistant to the conditions where drought and salinity is a common phenomenon. The aim of this study was to evaluate the diversity of rhizobial isolates obtained from root nodules of pigeon pea plants grown in central and southern Ethiopia. A total of 116 nitrogen-fixing rhizobial strains were isolated. The bacterial isolates were characterized by 91 phenotypic traits including cultural characteristics, intrinsic antibiotic and heavy metal resistance, salt, pH and incubation temperature tolerance, and carbon and nitrogen sources utilization ability. Preliminary symbiotic properties of the isolates were also evaluated. The isolates were compared with seven reference strains of rhizobia by application of the unweighted pair group method with arithmetic means (UPGMA) using NTSYSpc Version 2.1 software program. The dendrogram constructed from cluster analysis of 91 phenotypic traits, grouped them into six clusters and eight un-clustered positions at 80% relative similarity. Cluster I contained 83% of the test isolates that were grouped together with the reference strains Bradyrhizobium japonicum (HAMBI 2314T) and Bradyrhizobium elkanii (LMG 6164), suggesting that pigeon pea is commonly nodulated by bradyrhizobia. Results from symbiotic effectiveness test revealed that majority of the isolates were found to be effective. Generally, this investigation demonstrated that rhizobial population nodulating C. cajan on the study area were phenotypically diverse and symbiotically effective. Furthermore, the result indicates the existence of strains in the collection, which can tolerate environmental stresses, thus can be developed into inoculant for pigeon pea inoculation and production in Ethiopia and beyond. 2018-02-15 2018-03-20T13:41:34Z 2018-03-20T13:41:34Z Journal Article https://hdl.handle.net/10568/91713 en Open Access Academic Journals Degefu, T., Wolde-meskel, E., Adem, M., Fikre, A., Amede, T. and Ojiewo, C. 2018. Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia. African Journal of Biotechnology 17(6):167-177. |
| spellingShingle | plants pigeon peas research bradyrhizobium cajanus cajan rhizobium Degefu, T. Woldemeskel, Endalkachew Adem, M. Fikre, Asnake Amede, Tilahun Ojiewo, Christopher Ochieng Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia |
| title | Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia |
| title_full | Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia |
| title_fullStr | Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia |
| title_full_unstemmed | Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia |
| title_short | Morphophysiological diversity of rhizobia nodulating pigeon pea (Cajanus cajan L. Millsp.) growing in Ethiopia |
| title_sort | morphophysiological diversity of rhizobia nodulating pigeon pea cajanus cajan l millsp growing in ethiopia |
| topic | plants pigeon peas research bradyrhizobium cajanus cajan rhizobium |
| url | https://hdl.handle.net/10568/91713 |
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