Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America
Large quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava, us...
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1991
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/83251 |
| _version_ | 1855529051142225920 |
|---|---|
| author | Nolt, Barry L. Velasco, Ana Cecilia Pineda López, Benjamín |
| author_browse | Nolt, Barry L. Pineda López, Benjamín Velasco, Ana Cecilia |
| author_facet | Nolt, Barry L. Velasco, Ana Cecilia Pineda López, Benjamín |
| author_sort | Nolt, Barry L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Large quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava, using both the double-antibody sandwich or plate-trapped antigen forms of enzyme-linked immunosorbent assay (ELISA). The virus reacted with antisera to the potexviruses potato virus X and tulip virus X in F(ab`)2 ELISA. As determined by ELISA, isolates of CCMV from cassava and chaya are closely serologically related to each other. Leaf extracts from infected N. benthamiana plants were infective to a dilution of 10(- 4) but not 10(-5); after heating for 10 min at 65 degrees C but not 70 degrees C; and after storage at room temperature for 14 days. The virus has a sedimentation coefficient of 126 S20,w, a single coat protein molecule of c. mol. wt 21 000, and a single-stranded RNA genome of c. mol. wt of 2.0 x 10(6). Several dsRNA species, including the putative viral replicative form of c. mol. wt 4.1 x 10(6), were isolated from virus-infected cassava and N. benthamiana. (AS) |
| format | Journal Article |
| id | CGSpace83251 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1991 |
| publishDateRange | 1991 |
| publishDateSort | 1991 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace832512023-12-27T19:42:07Z Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America Nolt, Barry L. Velasco, Ana Cecilia Pineda López, Benjamín manihot esculenta cassava common mosaic virus purification elisa serology diseases and pathogens pests starch crops viroses virus del mosaico comun de la yuca purificacion serologia Large quantities of cassava common mosaic virus (CCMV) were purified from systemically infected Nicotiana benthamiana plants. A polyclonal antiserum, with a titre of 1/128 in the tube precipitin test, was produced by immunising rabbits with purified virus. Viral antigens were detected in cassava, using both the double-antibody sandwich or plate-trapped antigen forms of enzyme-linked immunosorbent assay (ELISA). The virus reacted with antisera to the potexviruses potato virus X and tulip virus X in F(ab`)2 ELISA. As determined by ELISA, isolates of CCMV from cassava and chaya are closely serologically related to each other. Leaf extracts from infected N. benthamiana plants were infective to a dilution of 10(- 4) but not 10(-5); after heating for 10 min at 65 degrees C but not 70 degrees C; and after storage at room temperature for 14 days. The virus has a sedimentation coefficient of 126 S20,w, a single coat protein molecule of c. mol. wt 21 000, and a single-stranded RNA genome of c. mol. wt of 2.0 x 10(6). Several dsRNA species, including the putative viral replicative form of c. mol. wt 4.1 x 10(6), were isolated from virus-infected cassava and N. benthamiana. (AS) 1991-02 2017-08-28T12:45:48Z 2017-08-28T12:45:48Z Journal Article https://hdl.handle.net/10568/83251 en Limited Access Wiley Nolt, B.L.; Velasco, A.C.; Pineda, B. 1991. Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America. Annals of Applied Biology 118(1):105-113. |
| spellingShingle | manihot esculenta cassava common mosaic virus purification elisa serology diseases and pathogens pests starch crops viroses virus del mosaico comun de la yuca purificacion serologia Nolt, Barry L. Velasco, Ana Cecilia Pineda López, Benjamín Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America |
| title | Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America |
| title_full | Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America |
| title_fullStr | Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America |
| title_full_unstemmed | Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America |
| title_short | Improved purification procedure and some serological and physical properties of cassava common mosaic virus from South America |
| title_sort | improved purification procedure and some serological and physical properties of cassava common mosaic virus from south america |
| topic | manihot esculenta cassava common mosaic virus purification elisa serology diseases and pathogens pests starch crops viroses virus del mosaico comun de la yuca purificacion serologia |
| url | https://hdl.handle.net/10568/83251 |
| work_keys_str_mv | AT noltbarryl improvedpurificationprocedureandsomeserologicalandphysicalpropertiesofcassavacommonmosaicvirusfromsouthamerica AT velascoanacecilia improvedpurificationprocedureandsomeserologicalandphysicalpropertiesofcassavacommonmosaicvirusfromsouthamerica AT pinedalopezbenjamin improvedpurificationprocedureandsomeserologicalandphysicalpropertiesofcassavacommonmosaicvirusfromsouthamerica |