A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, t...

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Bibliographic Details
Main Authors: Escobar Pérez, Roosevelt H., Mafla, G., Roca, W.
Format: Journal Article
Language:Inglés
Published: Springer 1997
Subjects:
Online Access:https://hdl.handle.net/10568/83250

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