A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, t...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
1997
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/83250 |
| _version_ | 1855531793053122560 |
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| author | Escobar Pérez, Roosevelt H. Mafla, G. Roca, W. |
| author_browse | Escobar Pérez, Roosevelt H. Mafla, G. Roca, W. |
| author_facet | Escobar Pérez, Roosevelt H. Mafla, G. Roca, W. |
| author_sort | Escobar Pérez, Roosevelt H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre- and post-freezing steps. Refining the methodology led to a consistent 50-70 percent plant recovery. (RA) |
| format | Journal Article |
| id | CGSpace83250 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace832502025-12-08T10:29:22Z A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen Escobar Pérez, Roosevelt H. Mafla, G. Roca, W. manihot esculenta biological conservation freezing mandioca conservación biológica congelación Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen, the shoot tips were removed, rapidly warmed, and recultured sequentially in three recovery media. After 2 weeks, the regeneration of frozen shoot tips was completed. Genotypes with a low response were identified. Their response was attributed to the effects of pre- and post-freezing steps. Refining the methodology led to a consistent 50-70 percent plant recovery. (RA) 1997 2017-08-28T12:45:47Z 2017-08-28T12:45:47Z Journal Article https://hdl.handle.net/10568/83250 en Limited Access Springer Escobar Pérez, Roosevelt H.; Mafla B., Graciela; Roca, William M. 1997. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen. Plant Cell Reports (Germany). 16(7):474-478. |
| spellingShingle | manihot esculenta biological conservation freezing mandioca conservación biológica congelación Escobar Pérez, Roosevelt H. Mafla, G. Roca, W. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| title | A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| title_full | A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| title_fullStr | A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| title_full_unstemmed | A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| title_short | A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| title_sort | methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen |
| topic | manihot esculenta biological conservation freezing mandioca conservación biológica congelación |
| url | https://hdl.handle.net/10568/83250 |
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