High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots
Past research developed reliable equations to base selections for high β-carotene on near-infrared spectroscopy (NIR) predictions (100 genotypes d−1) rather than with high-performance liquid chromatography (HPLC) (<10 samples d−1). During recent harvest, CIAT made selections based on NIR predictions...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2016
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/77289 |
| _version_ | 1855516488663826432 |
|---|---|
| author | Belalcázar, John Eiver Dufour, D.L. Andersson, Meike S. Pizarro, Mónica Luna, Jorge Londoño Hernandez, Luis Fernando Morante, Nelson Jaramillo Valencia, Angélica M. Pino, Lizbeth Becerra López Lavelle, Luis Augusto Davrieux, Fabrice Talsma, Elise F. Ceballos, H. |
| author_browse | Andersson, Meike S. Becerra López Lavelle, Luis Augusto Belalcázar, John Eiver Ceballos, H. Davrieux, Fabrice Dufour, D.L. Jaramillo Valencia, Angélica M. Londoño Hernandez, Luis Fernando Luna, Jorge Morante, Nelson Pino, Lizbeth Pizarro, Mónica Talsma, Elise F. |
| author_facet | Belalcázar, John Eiver Dufour, D.L. Andersson, Meike S. Pizarro, Mónica Luna, Jorge Londoño Hernandez, Luis Fernando Morante, Nelson Jaramillo Valencia, Angélica M. Pino, Lizbeth Becerra López Lavelle, Luis Augusto Davrieux, Fabrice Talsma, Elise F. Ceballos, H. |
| author_sort | Belalcázar, John Eiver |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Past research developed reliable equations to base selections for high β-carotene on near-infrared spectroscopy (NIR) predictions (100 genotypes d−1) rather than with high-performance liquid chromatography (HPLC) (<10 samples d−1). During recent harvest, CIAT made selections based on NIR predictions for the first time. This innovation produced valuable information that will help other cassava (Manihot esculenta Crantz) breeding programs. A total of 284 samples were analyzed with NIR and HPLC for total β-carotene (TBC) and by the oven method for dry matter content (DMC). Results indicated that NIR reliably predicted TBC and DMC. In addition, 232 genotypes grown in preliminary yield trials (PYTs) were harvested at 8.5 and 10.5 mo after planting (one plant per genotype and age) and root quality traits analyzed (by NIR only). Repeatability of results at the two ages was excellent, suggesting reliable results from NIR. In contrast to previous reports, age of the plant did not influence carotenoids content in the roots. The availability of a high-throughput NIR protocol allowed comparing results (for the first time) from seedling and cloned plants from the same genotype. Results showed very little relationship for DMC between seedling and cloned plants (R2 = 0.09). There was a much better association for TBC (R2 = 0.48) between seedling and cloned plants. It is postulated that variation in the environmental conditions when seedling and cloned plants (from the same genotype) may be responsible for these weak associations. Important changes in selection strategies have been implemented to overcome problems related to a lengthy harvesting season. |
| format | Journal Article |
| id | CGSpace77289 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace772892025-03-13T09:44:00Z High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots Belalcázar, John Eiver Dufour, D.L. Andersson, Meike S. Pizarro, Mónica Luna, Jorge Londoño Hernandez, Luis Fernando Morante, Nelson Jaramillo Valencia, Angélica M. Pino, Lizbeth Becerra López Lavelle, Luis Augusto Davrieux, Fabrice Talsma, Elise F. Ceballos, H. manihot esculenta plant breeding carotenoids spectroscopy genotypes fitomejoramiento carotenoides espectroscopia marcadores genéticos Past research developed reliable equations to base selections for high β-carotene on near-infrared spectroscopy (NIR) predictions (100 genotypes d−1) rather than with high-performance liquid chromatography (HPLC) (<10 samples d−1). During recent harvest, CIAT made selections based on NIR predictions for the first time. This innovation produced valuable information that will help other cassava (Manihot esculenta Crantz) breeding programs. A total of 284 samples were analyzed with NIR and HPLC for total β-carotene (TBC) and by the oven method for dry matter content (DMC). Results indicated that NIR reliably predicted TBC and DMC. In addition, 232 genotypes grown in preliminary yield trials (PYTs) were harvested at 8.5 and 10.5 mo after planting (one plant per genotype and age) and root quality traits analyzed (by NIR only). Repeatability of results at the two ages was excellent, suggesting reliable results from NIR. In contrast to previous reports, age of the plant did not influence carotenoids content in the roots. The availability of a high-throughput NIR protocol allowed comparing results (for the first time) from seedling and cloned plants from the same genotype. Results showed very little relationship for DMC between seedling and cloned plants (R2 = 0.09). There was a much better association for TBC (R2 = 0.48) between seedling and cloned plants. It is postulated that variation in the environmental conditions when seedling and cloned plants (from the same genotype) may be responsible for these weak associations. Important changes in selection strategies have been implemented to overcome problems related to a lengthy harvesting season. 2016-11 2016-10-13T15:24:24Z 2016-10-13T15:24:24Z Journal Article https://hdl.handle.net/10568/77289 en Open Access Wiley Belalcazar, John; Dufour, Dominique; Andersson, Meike S.; Pizarro, Mónica; Luna, Jorge; Londoño, Luis; Morante, Nelson; Jaramillo, Angélica M.; Pino, Lizbeth; Becerra López-Lavalle, Luis Augusto; Davrieux, Fabrice; Talsma, Elise F.; Ceballos, Hernán. 2016. High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots . Crop Science 56 (6): 2916-2928. |
| spellingShingle | manihot esculenta plant breeding carotenoids spectroscopy genotypes fitomejoramiento carotenoides espectroscopia marcadores genéticos Belalcázar, John Eiver Dufour, D.L. Andersson, Meike S. Pizarro, Mónica Luna, Jorge Londoño Hernandez, Luis Fernando Morante, Nelson Jaramillo Valencia, Angélica M. Pino, Lizbeth Becerra López Lavelle, Luis Augusto Davrieux, Fabrice Talsma, Elise F. Ceballos, H. High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| title | High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| title_full | High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| title_fullStr | High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| title_full_unstemmed | High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| title_short | High-throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| title_sort | high throughput phenotyping and improvements in breeding cassava for increased carotenoids in the roots |
| topic | manihot esculenta plant breeding carotenoids spectroscopy genotypes fitomejoramiento carotenoides espectroscopia marcadores genéticos |
| url | https://hdl.handle.net/10568/77289 |
| work_keys_str_mv | AT belalcazarjohneiver highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT dufourdl highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT anderssonmeikes highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT pizarromonica highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT lunajorge highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT londonohernandezluisfernando highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT morantenelson highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT jaramillovalenciaangelicam highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT pinolizbeth highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT becerralopezlavelleluisaugusto highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT davrieuxfabrice highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT talsmaelisef highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots AT ceballosh highthroughputphenotypingandimprovementsinbreedingcassavaforincreasedcarotenoidsintheroots |