Isolation and identification of Listeria species along the milk value chain in one region of Tanzania

Milk is an important source of nutrients in the diet. However, due to its nutritional nature it also serves as an ideal medium for growth and development of various microorganisms under suitable conditions. It is a staple food, which is linked to zoonotic pathogens. This study was carried out in...

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Main Authors: Hyera, E., Msalya, G., Karimuribo, E.D., Kurwijila, Lusato R., Alonso, Silvia, Roesel, Kristina, Grace, Delia
Format: Poster
Language:Inglés
Published: Tanzania Livestock Research Institute 2016
Subjects:
Online Access:https://hdl.handle.net/10568/77092
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author Hyera, E.
Msalya, G.
Karimuribo, E.D.
Kurwijila, Lusato R.
Alonso, Silvia
Roesel, Kristina
Grace, Delia
author_browse Alonso, Silvia
Grace, Delia
Hyera, E.
Karimuribo, E.D.
Kurwijila, Lusato R.
Msalya, G.
Roesel, Kristina
author_facet Hyera, E.
Msalya, G.
Karimuribo, E.D.
Kurwijila, Lusato R.
Alonso, Silvia
Roesel, Kristina
Grace, Delia
author_sort Hyera, E.
collection Repository of Agricultural Research Outputs (CGSpace)
description Milk is an important source of nutrients in the diet. However, due to its nutritional nature it also serves as an ideal medium for growth and development of various microorganisms under suitable conditions. It is a staple food, which is linked to zoonotic pathogens. This study was carried out in two districts (one region namely Tanga) of Tanzania to evaluate the awareness of farmers on milk contamination and to estimate microbial load along the milk value chain. In total 114 respondents were visited to conducted interviews on awareness as well as strategies for solving the problem of milk contamination. Subsequently milk samples were aseptically collected from each of the 114 respondents for laboratory analyses using the standard ISO procedures for food microbial analyses in particular the horizontal methods. We have showed that there are poor hygienic practices by the majority of actors along the milk value chain in Tanga region. Concerning microbial level, our results revealed that above 90 percent of all samples collected showed total plate count (TPC) above standard acceptable in the Eastern Africa countries (EAC) of 2.0x105 CFU/ml. It was also shown that there was more contamination in milk samples obtained from the household farms compared to those which were collected from the milk suppliers and in the extensive farming system than in the intensive system (P < 0.05). Moreover, three Listeria species namely L. innocua, L. ivanovii and L. monocytogenes were identified. L. monocytogenes were isolated and identified as the common contaminants in milk along the value chain in the study region.
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spelling CGSpace770922025-11-04T17:35:59Z Isolation and identification of Listeria species along the milk value chain in one region of Tanzania Hyera, E. Msalya, G. Karimuribo, E.D. Kurwijila, Lusato R. Alonso, Silvia Roesel, Kristina Grace, Delia dairies food safety Milk is an important source of nutrients in the diet. However, due to its nutritional nature it also serves as an ideal medium for growth and development of various microorganisms under suitable conditions. It is a staple food, which is linked to zoonotic pathogens. This study was carried out in two districts (one region namely Tanga) of Tanzania to evaluate the awareness of farmers on milk contamination and to estimate microbial load along the milk value chain. In total 114 respondents were visited to conducted interviews on awareness as well as strategies for solving the problem of milk contamination. Subsequently milk samples were aseptically collected from each of the 114 respondents for laboratory analyses using the standard ISO procedures for food microbial analyses in particular the horizontal methods. We have showed that there are poor hygienic practices by the majority of actors along the milk value chain in Tanga region. Concerning microbial level, our results revealed that above 90 percent of all samples collected showed total plate count (TPC) above standard acceptable in the Eastern Africa countries (EAC) of 2.0x105 CFU/ml. It was also shown that there was more contamination in milk samples obtained from the household farms compared to those which were collected from the milk suppliers and in the extensive farming system than in the intensive system (P < 0.05). Moreover, three Listeria species namely L. innocua, L. ivanovii and L. monocytogenes were identified. L. monocytogenes were isolated and identified as the common contaminants in milk along the value chain in the study region. 2016-09-04 2016-09-20T10:20:24Z 2016-09-20T10:20:24Z Poster https://hdl.handle.net/10568/77092 en Open Access application/pdf Tanzania Livestock Research Institute Hyera, E., Msalya, G., Karimuribo, E.D., Kurwijila, L.R., Alonso, S., Roesel, K. and Grace, D. 2016. Isolation and identification of Listeria species along the milk value chain in one region of Tanzania. Poster presented at the first joint conference of the Association of Institutions for Tropical Veterinary Medicine and the Society of Tropical Veterinary Medicine, Berlin, Germany, 4–8 September 2016. Kilimanjaro, Tanzania: Tanzania Livestock Research Institute.
spellingShingle dairies
food safety
Hyera, E.
Msalya, G.
Karimuribo, E.D.
Kurwijila, Lusato R.
Alonso, Silvia
Roesel, Kristina
Grace, Delia
Isolation and identification of Listeria species along the milk value chain in one region of Tanzania
title Isolation and identification of Listeria species along the milk value chain in one region of Tanzania
title_full Isolation and identification of Listeria species along the milk value chain in one region of Tanzania
title_fullStr Isolation and identification of Listeria species along the milk value chain in one region of Tanzania
title_full_unstemmed Isolation and identification of Listeria species along the milk value chain in one region of Tanzania
title_short Isolation and identification of Listeria species along the milk value chain in one region of Tanzania
title_sort isolation and identification of listeria species along the milk value chain in one region of tanzania
topic dairies
food safety
url https://hdl.handle.net/10568/77092
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