Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda
Since the 1980s, pork has become very popular in eastern Africa, with Uganda currently leading per capita consumption. Most of the pork is produced locally by smallholder pig farmers who ventured into piggery as a profitable income–generating activity. Yet, knowledge of good husbandry practices i...
| Autores principales: | , , , , , , |
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| Formato: | Conference Paper |
| Lenguaje: | Inglés |
| Publicado: |
2015
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| Acceso en línea: | https://hdl.handle.net/10568/68510 |
| _version_ | 1855520411130789888 |
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| author | Roesel, Kristina Dione, Michel M. Nöckler, K. Fries, R. Baumann, M.P.O. Clausen, Peter-Henning Grace, Delia |
| author_browse | Baumann, M.P.O. Clausen, Peter-Henning Dione, Michel M. Fries, R. Grace, Delia Nöckler, K. Roesel, Kristina |
| author_facet | Roesel, Kristina Dione, Michel M. Nöckler, K. Fries, R. Baumann, M.P.O. Clausen, Peter-Henning Grace, Delia |
| author_sort | Roesel, Kristina |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Since the 1980s, pork has become very popular in eastern Africa, with Uganda currently
leading per capita consumption. Most of the pork is produced locally by smallholder pig
farmers who ventured into piggery as a profitable income–generating activity. Yet, knowledge
of good husbandry practices is limited and the majority of the pigs are kept in systems that
allow free–ranging and scavenging. Nematodes of the Trichinella genus are known to enter
the human food chain through the consumption of undercooked pork. In the East African
Community, data on the presence of Trichinella spp. in domestic pigs is scarce and limited to
erratic surveys using diagnostic methods with a low sensitivity such as trichinoscopy. This
study aimed to determine if the domestic cycle of the parasite plays a role in Ugandan pigs
and if consumers are at risk of contracting trichinellosis from eating undercooked pork.
In a cross–sectional survey conducted from April to July 2013 we sampled more than 1,000
smallholder pig farms in three districts in Central and Eastern Uganda. As part of a multi–
pathogen assessment we collected pig sera, bio–data of the individual animals, herd
composition and husbandry practices at production. The sera were examined using a
commercially available enzyme–linked immunosorbant assay detecting anti–Trichinella–IgG.
Positive samples underwent Western Blot for confirmation.
Seven percent (80/1124) of the sera tested positive and 97.5 % of these sero–positives
originated from rural production systems. Only one third of these were confirmed IgG positive
in the Western blot. Subsequently, 500 pork meat samples from four geographical clusters
with a high seroprevalence were collected from November to December 2014 and examined
using the artificial digestion method. All samples were negative for Trichinella larvae.
The presentation will discuss the implications of a sensitivity analysis, potential reasons for
the high number of false–positives using the commercial ELISA as well as the suitability of
indirect serological diagnostic tools developed in industrialized countries for utilization in
extensive production systems in tropical countries. |
| format | Conference Paper |
| id | CGSpace68510 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| record_format | dspace |
| spelling | CGSpace685102024-03-06T10:16:43Z Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda Roesel, Kristina Dione, Michel M. Nöckler, K. Fries, R. Baumann, M.P.O. Clausen, Peter-Henning Grace, Delia Since the 1980s, pork has become very popular in eastern Africa, with Uganda currently leading per capita consumption. Most of the pork is produced locally by smallholder pig farmers who ventured into piggery as a profitable income–generating activity. Yet, knowledge of good husbandry practices is limited and the majority of the pigs are kept in systems that allow free–ranging and scavenging. Nematodes of the Trichinella genus are known to enter the human food chain through the consumption of undercooked pork. In the East African Community, data on the presence of Trichinella spp. in domestic pigs is scarce and limited to erratic surveys using diagnostic methods with a low sensitivity such as trichinoscopy. This study aimed to determine if the domestic cycle of the parasite plays a role in Ugandan pigs and if consumers are at risk of contracting trichinellosis from eating undercooked pork. In a cross–sectional survey conducted from April to July 2013 we sampled more than 1,000 smallholder pig farms in three districts in Central and Eastern Uganda. As part of a multi– pathogen assessment we collected pig sera, bio–data of the individual animals, herd composition and husbandry practices at production. The sera were examined using a commercially available enzyme–linked immunosorbant assay detecting anti–Trichinella–IgG. Positive samples underwent Western Blot for confirmation. Seven percent (80/1124) of the sera tested positive and 97.5 % of these sero–positives originated from rural production systems. Only one third of these were confirmed IgG positive in the Western blot. Subsequently, 500 pork meat samples from four geographical clusters with a high seroprevalence were collected from November to December 2014 and examined using the artificial digestion method. All samples were negative for Trichinella larvae. The presentation will discuss the implications of a sensitivity analysis, potential reasons for the high number of false–positives using the commercial ELISA as well as the suitability of indirect serological diagnostic tools developed in industrialized countries for utilization in extensive production systems in tropical countries. 2015-09 2015-10-13T12:26:40Z 2015-10-13T12:26:40Z Conference Paper https://hdl.handle.net/10568/68510 en Limited Access Roesel, K., Dione, M., Nöckler, K., Fries, R., Baumann, M.P.O., Clausen, P.-H. and Grace, D. 2015. Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda. Abstract of paper presented at the 14th International Conference on Trichinellosis, Berlin, Germany, 14-18 September 2015. |
| spellingShingle | Roesel, Kristina Dione, Michel M. Nöckler, K. Fries, R. Baumann, M.P.O. Clausen, Peter-Henning Grace, Delia Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda |
| title | Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda |
| title_full | Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda |
| title_fullStr | Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda |
| title_full_unstemmed | Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda |
| title_short | Exposure of pigs to Trichinella spp. in three districts in Central and Eastern Uganda |
| title_sort | exposure of pigs to trichinella spp in three districts in central and eastern uganda |
| url | https://hdl.handle.net/10568/68510 |
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