Sampling strategies for proper quantification of carotenoid content in cassava breeding
Rapid-cycling recurrent selection has been implemented to produce cassava varieties that deliver provitamin A carotenoids to human populations that are affected by vitamin A deficiency. Rapid-cycling selection relies on quantification in just one plant per genotype. This study was conducted to ass...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2011
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/44068 |
| _version_ | 1855523985498832896 |
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| author | Ortíz, D. Sánchez, T. Morante, N Ceballos, H. Pachón, Helena Duque E., Myriam Cristina Chavez, Alba L. Escobar Salamanca, Andrés F. |
| author_browse | Ceballos, H. Chavez, Alba L. Duque E., Myriam Cristina Escobar Salamanca, Andrés F. Morante, N Ortíz, D. Pachón, Helena Sánchez, T. |
| author_facet | Ortíz, D. Sánchez, T. Morante, N Ceballos, H. Pachón, Helena Duque E., Myriam Cristina Chavez, Alba L. Escobar Salamanca, Andrés F. |
| author_sort | Ortíz, D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rapid-cycling recurrent selection has been implemented to produce cassava varieties that deliver provitamin A carotenoids to human populations that are affected by vitamin A deficiency. Rapid-cycling
selection relies on quantification in just one plant per genotype. This study was conducted to assess the
reliability of single-plant evaluations and the effect of age of the plant on carotenoid content in cassava
roots. Variation in aliquot quantifications from the same root was negligible indicating a reliable
experimental procedure. A large source of variation for carotenoids was due to differences among
genotypes. Root-to-root variation from the same plant in some cases was surprisingly high and
accounted for an average of 25% of the total variation. Plant-to-plant variation was not as high and
accounted for 20% of the total variance. Carotenoid content was shown to vary depending on the age of
the plant. Single-plant evaluations for carotenoid content in cassava, which is a requirement for rapidcycling recurrent selection is acceptable considering that it reduces in half the time required for
evaluation and selection. However, it is suggested that 2 to 3 roots per plant be combined together in a
sample to represent each genotype at a standard plant age. |
| format | Journal Article |
| id | CGSpace44068 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| record_format | dspace |
| spelling | CGSpace440682024-03-06T10:16:43Z Sampling strategies for proper quantification of carotenoid content in cassava breeding Ortíz, D. Sánchez, T. Morante, N Ceballos, H. Pachón, Helena Duque E., Myriam Cristina Chavez, Alba L. Escobar Salamanca, Andrés F. manihot esculenta genetic gain carotenoids varieties vitamin deficiencies genotypes mejora genética carotenoides variedades deficiencia de vitaminas genotipos Rapid-cycling recurrent selection has been implemented to produce cassava varieties that deliver provitamin A carotenoids to human populations that are affected by vitamin A deficiency. Rapid-cycling selection relies on quantification in just one plant per genotype. This study was conducted to assess the reliability of single-plant evaluations and the effect of age of the plant on carotenoid content in cassava roots. Variation in aliquot quantifications from the same root was negligible indicating a reliable experimental procedure. A large source of variation for carotenoids was due to differences among genotypes. Root-to-root variation from the same plant in some cases was surprisingly high and accounted for an average of 25% of the total variation. Plant-to-plant variation was not as high and accounted for 20% of the total variance. Carotenoid content was shown to vary depending on the age of the plant. Single-plant evaluations for carotenoid content in cassava, which is a requirement for rapidcycling recurrent selection is acceptable considering that it reduces in half the time required for evaluation and selection. However, it is suggested that 2 to 3 roots per plant be combined together in a sample to represent each genotype at a standard plant age. 2011 2014-10-02T08:33:11Z 2014-10-02T08:33:11Z Journal Article https://hdl.handle.net/10568/44068 en Open Access |
| spellingShingle | manihot esculenta genetic gain carotenoids varieties vitamin deficiencies genotypes mejora genética carotenoides variedades deficiencia de vitaminas genotipos Ortíz, D. Sánchez, T. Morante, N Ceballos, H. Pachón, Helena Duque E., Myriam Cristina Chavez, Alba L. Escobar Salamanca, Andrés F. Sampling strategies for proper quantification of carotenoid content in cassava breeding |
| title | Sampling strategies for proper quantification of carotenoid content in cassava breeding |
| title_full | Sampling strategies for proper quantification of carotenoid content in cassava breeding |
| title_fullStr | Sampling strategies for proper quantification of carotenoid content in cassava breeding |
| title_full_unstemmed | Sampling strategies for proper quantification of carotenoid content in cassava breeding |
| title_short | Sampling strategies for proper quantification of carotenoid content in cassava breeding |
| title_sort | sampling strategies for proper quantification of carotenoid content in cassava breeding |
| topic | manihot esculenta genetic gain carotenoids varieties vitamin deficiencies genotypes mejora genética carotenoides variedades deficiencia de vitaminas genotipos |
| url | https://hdl.handle.net/10568/44068 |
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