Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates
Two monoclonal antibodies prepared to purified virions of bean golden mosaic virus (BGMV) isolates from Guatemala (GA) and the Dominican Republic (DR) and one monoclonal antibody prepared to the coat protein of a BGMV isolate from Brazil (BZ) expressed in Escherichia coli were useful serological pro...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Societies
1995
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43478 |
| _version_ | 1855519489037172736 |
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| author | Cancino, M Abouzid, AM Morales, Francisco José Purcifull, DE Polston, JE Hiebert, E |
| author_browse | Abouzid, AM Cancino, M Hiebert, E Morales, Francisco José Polston, JE Purcifull, DE |
| author_facet | Cancino, M Abouzid, AM Morales, Francisco José Purcifull, DE Polston, JE Hiebert, E |
| author_sort | Cancino, M |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two monoclonal antibodies prepared to purified virions of bean golden mosaic virus (BGMV) isolates from Guatemala (GA) and the Dominican Republic (DR) and one monoclonal antibody prepared to the coat protein of a BGMV isolate from Brazil (BZ) expressed in Escherichia coli were useful serological probes for detection of whitefly-transmitted geminiviruses. Abutilon mosaic, BGMV isolates from Puerto Rico (PR), and Homestead, Florida (H), BGMV-BZ, -DR, and -GA isolates, euphorbia mosaic, rhynchosia mosaic, squash leaf curl, soybean yellow mosaic, an isolate from Macroptilium lathyroides in Florida, and tomato mottle geminiviruses all reacted efficiently in Western blot and enzyme-linked immunosorbent assay with monoclonal antibody 3F7, indicating that this monoclonal antibody has a broad spectrum of reactivity. Another monoclonal antibody (2G5) reacted only with BGMV-DR, -GA, and -PR isolates. A third monoclonal antibody (5C5) reacted only with BGMV-BZ. A new geminivirus infecting cabbage (Brassicaceae) in Florida did not react with any of the three monoclonal antibodies. |
| format | Journal Article |
| id | CGSpace43478 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace434782024-05-01T08:16:00Z Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates Cancino, M Abouzid, AM Morales, Francisco José Purcifull, DE Polston, JE Hiebert, E phaseolus vulgaris geminiviruses testa monoclonal antibodies nucleotide sequence geminivirus anticuerpos monoclonales secuencia nucleotídica Two monoclonal antibodies prepared to purified virions of bean golden mosaic virus (BGMV) isolates from Guatemala (GA) and the Dominican Republic (DR) and one monoclonal antibody prepared to the coat protein of a BGMV isolate from Brazil (BZ) expressed in Escherichia coli were useful serological probes for detection of whitefly-transmitted geminiviruses. Abutilon mosaic, BGMV isolates from Puerto Rico (PR), and Homestead, Florida (H), BGMV-BZ, -DR, and -GA isolates, euphorbia mosaic, rhynchosia mosaic, squash leaf curl, soybean yellow mosaic, an isolate from Macroptilium lathyroides in Florida, and tomato mottle geminiviruses all reacted efficiently in Western blot and enzyme-linked immunosorbent assay with monoclonal antibody 3F7, indicating that this monoclonal antibody has a broad spectrum of reactivity. Another monoclonal antibody (2G5) reacted only with BGMV-DR, -GA, and -PR isolates. A third monoclonal antibody (5C5) reacted only with BGMV-BZ. A new geminivirus infecting cabbage (Brassicaceae) in Florida did not react with any of the three monoclonal antibodies. 1995 2014-09-24T08:42:11Z 2014-09-24T08:42:11Z Journal Article https://hdl.handle.net/10568/43478 en Open Access Scientific Societies |
| spellingShingle | phaseolus vulgaris geminiviruses testa monoclonal antibodies nucleotide sequence geminivirus anticuerpos monoclonales secuencia nucleotídica Cancino, M Abouzid, AM Morales, Francisco José Purcifull, DE Polston, JE Hiebert, E Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| title | Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| title_full | Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| title_fullStr | Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| title_full_unstemmed | Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| title_short | Generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| title_sort | generation and characterization of three monoclonal antibodies useful in detecting and distinguishing bean golden mosaic virus isolates |
| topic | phaseolus vulgaris geminiviruses testa monoclonal antibodies nucleotide sequence geminivirus anticuerpos monoclonales secuencia nucleotídica |
| url | https://hdl.handle.net/10568/43478 |
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