Addressing the dilemmas of measuring amylose in rice

Amylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorba...

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Autores principales: Fitzgerald, MA, Bergman, C.J., Resurreccion, AP, Moller, J, Jiménez, R., Reinke, RF, Martin, M., Blanco, P., Molina, F., Chen, M.H., Kuri, Victoria Eugenia, Romero, M.V., Habibi, F, Umemoto, Takayuki, Jongdee, Supanee, Graterol Matute, Eduardo Jose, Reddy, KR, Bassinello, P. Z., Sivakami, R, Rani, NS, Das, S., Wang, YJ, Indrasari, S.D., Ramli, A., Ahmad, Rauf, Dipti, S.S., Xie, L, Nguyen, TL, Singh, P., Castillo Toro, D., Tavasoli, F., Mestres, C.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2009
Materias:
Acceso en línea:https://hdl.handle.net/10568/42551
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author Fitzgerald, MA
Bergman, C.J.
Resurreccion, AP
Moller, J
Jiménez, R.
Reinke, RF
Martin, M.
Blanco, P.
Molina, F.
Chen, M.H.
Kuri, Victoria Eugenia
Romero, M.V.
Habibi, F
Umemoto, Takayuki
Jongdee, Supanee
Graterol Matute, Eduardo Jose
Reddy, KR
Bassinello, P. Z.
Sivakami, R
Rani, NS
Das, S.
Wang, YJ
Indrasari, S.D.
Ramli, A.
Ahmad, Rauf
Dipti, S.S.
Xie, L
Nguyen, TL
Singh, P.
Castillo Toro, D.
Tavasoli, F.
Mestres, C.
author_browse Ahmad, Rauf
Bassinello, P. Z.
Bergman, C.J.
Blanco, P.
Castillo Toro, D.
Chen, M.H.
Das, S.
Dipti, S.S.
Fitzgerald, MA
Graterol Matute, Eduardo Jose
Habibi, F
Indrasari, S.D.
Jiménez, R.
Jongdee, Supanee
Kuri, Victoria Eugenia
Martin, M.
Mestres, C.
Molina, F.
Moller, J
Nguyen, TL
Ramli, A.
Rani, NS
Reddy, KR
Reinke, RF
Resurreccion, AP
Romero, M.V.
Singh, P.
Sivakami, R
Tavasoli, F.
Umemoto, Takayuki
Wang, YJ
Xie, L
author_facet Fitzgerald, MA
Bergman, C.J.
Resurreccion, AP
Moller, J
Jiménez, R.
Reinke, RF
Martin, M.
Blanco, P.
Molina, F.
Chen, M.H.
Kuri, Victoria Eugenia
Romero, M.V.
Habibi, F
Umemoto, Takayuki
Jongdee, Supanee
Graterol Matute, Eduardo Jose
Reddy, KR
Bassinello, P. Z.
Sivakami, R
Rani, NS
Das, S.
Wang, YJ
Indrasari, S.D.
Ramli, A.
Ahmad, Rauf
Dipti, S.S.
Xie, L
Nguyen, TL
Singh, P.
Castillo Toro, D.
Tavasoli, F.
Mestres, C.
author_sort Fitzgerald, MA
collection Repository of Agricultural Research Outputs (CGSpace)
description Amylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorbance of the amylose‐iodine complex. The International Network for Quality Rice (INQR) conducted a survey to determine ways that amylose is measured, reproducibility between laboratories, and sources of variation. Each laboratory measured the amylose content of a set of 17 cultivars of rice. The study shows that five different versions of the iodine binding method are in use. The data show that repeatability was high within laboratories but reproducibility between laboratories was low. The major sources of variability were the way the standard curve was constructed and the iodine binding capacity of the potato amylose used to produce the standard. Reproducibility is much lower between laboratories using a standard curve of potato amylose alone compared with those using calibrated rice cultivars. This study highlights the need to standardize the way amylose is measured, and presents research avenues for doing so.
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spelling CGSpace425512024-08-27T10:35:34Z Addressing the dilemmas of measuring amylose in rice Fitzgerald, MA Bergman, C.J. Resurreccion, AP Moller, J Jiménez, R. Reinke, RF Martin, M. Blanco, P. Molina, F. Chen, M.H. Kuri, Victoria Eugenia Romero, M.V. Habibi, F Umemoto, Takayuki Jongdee, Supanee Graterol Matute, Eduardo Jose Reddy, KR Bassinello, P. Z. Sivakami, R Rani, NS Das, S. Wang, YJ Indrasari, S.D. Ramli, A. Ahmad, Rauf Dipti, S.S. Xie, L Nguyen, TL Singh, P. Castillo Toro, D. Tavasoli, F. Mestres, C. oryza sativa rice amylose arroz amilosa Amylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorbance of the amylose‐iodine complex. The International Network for Quality Rice (INQR) conducted a survey to determine ways that amylose is measured, reproducibility between laboratories, and sources of variation. Each laboratory measured the amylose content of a set of 17 cultivars of rice. The study shows that five different versions of the iodine binding method are in use. The data show that repeatability was high within laboratories but reproducibility between laboratories was low. The major sources of variability were the way the standard curve was constructed and the iodine binding capacity of the potato amylose used to produce the standard. Reproducibility is much lower between laboratories using a standard curve of potato amylose alone compared with those using calibrated rice cultivars. This study highlights the need to standardize the way amylose is measured, and presents research avenues for doing so. 2009-09 2014-09-24T07:58:11Z 2014-09-24T07:58:11Z Journal Article https://hdl.handle.net/10568/42551 en Limited Access Wiley
spellingShingle oryza sativa
rice
amylose
arroz
amilosa
Fitzgerald, MA
Bergman, C.J.
Resurreccion, AP
Moller, J
Jiménez, R.
Reinke, RF
Martin, M.
Blanco, P.
Molina, F.
Chen, M.H.
Kuri, Victoria Eugenia
Romero, M.V.
Habibi, F
Umemoto, Takayuki
Jongdee, Supanee
Graterol Matute, Eduardo Jose
Reddy, KR
Bassinello, P. Z.
Sivakami, R
Rani, NS
Das, S.
Wang, YJ
Indrasari, S.D.
Ramli, A.
Ahmad, Rauf
Dipti, S.S.
Xie, L
Nguyen, TL
Singh, P.
Castillo Toro, D.
Tavasoli, F.
Mestres, C.
Addressing the dilemmas of measuring amylose in rice
title Addressing the dilemmas of measuring amylose in rice
title_full Addressing the dilemmas of measuring amylose in rice
title_fullStr Addressing the dilemmas of measuring amylose in rice
title_full_unstemmed Addressing the dilemmas of measuring amylose in rice
title_short Addressing the dilemmas of measuring amylose in rice
title_sort addressing the dilemmas of measuring amylose in rice
topic oryza sativa
rice
amylose
arroz
amilosa
url https://hdl.handle.net/10568/42551
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