Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one in Cen...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2005
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33216 |
| _version_ | 1855534068441022464 |
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| author | Musoke, A.J. Rowlands, G.J. Nene, Vishvanath M. Nyanjui, J. Katende, J. Spooner, P.R. Mwaura, S. Odongo, D. Nkonge, C. Mbogo, S. Bishop, Richard P. Morzaria, S.P. |
| author_browse | Bishop, Richard P. Katende, J. Mbogo, S. Morzaria, S.P. Musoke, A.J. Mwaura, S. Nene, Vishvanath M. Nkonge, C. Nyanjui, J. Odongo, D. Rowlands, G.J. Spooner, P.R. |
| author_facet | Musoke, A.J. Rowlands, G.J. Nene, Vishvanath M. Nyanjui, J. Katende, J. Spooner, P.R. Mwaura, S. Odongo, D. Nkonge, C. Mbogo, S. Bishop, Richard P. Morzaria, S.P. |
| author_sort | Musoke, A.J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one in Central Kenya. Vaccination reduced severe ECF by 47% at the coast and by 52% in central Kenya from an average incidence of 0.53 [plus-or-minus sign] 0.07 (S.E.) in 50 non-immunised controls to an average of 0.27 [plus-or-minus sign] 0.05 in 83 immunised animals. The reduction in severe East Coast fever was similar to that observed in laboratory experiments with p67635 and p67C. The p67 coding sequence from thirteen T. parva field isolates including seven from vaccinated cattle that were not protected, was 100% identical to the gene on which the recombinant vaccine is based, suggesting a predominantly homologous p67 antigenic challenge. The same parasite isolates were however genetically heterogeneous at several loci other than p67. |
| format | Journal Article |
| id | CGSpace33216 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace332162024-01-17T12:58:34Z Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge Musoke, A.J. Rowlands, G.J. Nene, Vishvanath M. Nyanjui, J. Katende, J. Spooner, P.R. Mwaura, S. Odongo, D. Nkonge, C. Mbogo, S. Bishop, Richard P. Morzaria, S.P. east coast fever synthetic vaccines theileria parva sporozoa infection cattle immunization antibodies dna monoclonal antibodies polymorphism cloning parasites Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one in Central Kenya. Vaccination reduced severe ECF by 47% at the coast and by 52% in central Kenya from an average incidence of 0.53 [plus-or-minus sign] 0.07 (S.E.) in 50 non-immunised controls to an average of 0.27 [plus-or-minus sign] 0.05 in 83 immunised animals. The reduction in severe East Coast fever was similar to that observed in laboratory experiments with p67635 and p67C. The p67 coding sequence from thirteen T. parva field isolates including seven from vaccinated cattle that were not protected, was 100% identical to the gene on which the recombinant vaccine is based, suggesting a predominantly homologous p67 antigenic challenge. The same parasite isolates were however genetically heterogeneous at several loci other than p67. 2005-04 2013-07-03T05:26:14Z 2013-07-03T05:26:14Z Journal Article https://hdl.handle.net/10568/33216 en Limited Access Elsevier Vaccine;23(23): 3084-3095 |
| spellingShingle | east coast fever synthetic vaccines theileria parva sporozoa infection cattle immunization antibodies dna monoclonal antibodies polymorphism cloning parasites Musoke, A.J. Rowlands, G.J. Nene, Vishvanath M. Nyanjui, J. Katende, J. Spooner, P.R. Mwaura, S. Odongo, D. Nkonge, C. Mbogo, S. Bishop, Richard P. Morzaria, S.P. Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| title | Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| title_full | Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| title_fullStr | Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| title_full_unstemmed | Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| title_short | Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| title_sort | subunit vaccine based on the p67 major surface protein of theileria parva sporozoites reduces severity of infection derived from field tick challenge |
| topic | east coast fever synthetic vaccines theileria parva sporozoa infection cattle immunization antibodies dna monoclonal antibodies polymorphism cloning parasites |
| url | https://hdl.handle.net/10568/33216 |
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