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A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiven...

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Main Authors: Ballingall, Keith T., Mwangi, David Miano, MacHugh, Niall D., Taracha, E.L.N., Totte, P., McKeever, Declan J.
Format: Journal Article
Language:Inglés
Published: Elsevier 2000
Subjects:
cattle
antigens
bovidae
cells
antibodies
theileria parva
Online Access:https://hdl.handle.net/10568/33010
Summary:We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN y and activation of immune CD4 - and CD8 - T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4-T cell clones.

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