A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiven...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2000
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33010 |
| _version_ | 1855543014621970432 |
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| author | Ballingall, Keith T. Mwangi, David Miano MacHugh, Niall D. Taracha, E.L.N. Totte, P. McKeever, Declan J. |
| author_browse | Ballingall, Keith T. MacHugh, Niall D. McKeever, Declan J. Mwangi, David Miano Taracha, E.L.N. Totte, P. |
| author_facet | Ballingall, Keith T. Mwangi, David Miano MacHugh, Niall D. Taracha, E.L.N. Totte, P. McKeever, Declan J. |
| author_sort | Ballingall, Keith T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN y and activation of immune CD4 - and CD8 - T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4-T cell clones. |
| format | Journal Article |
| id | CGSpace33010 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace330102024-05-01T08:20:06Z A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells Ballingall, Keith T. Mwangi, David Miano MacHugh, Niall D. Taracha, E.L.N. Totte, P. McKeever, Declan J. cattle antigens bovidae cells antibodies theileria parva We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN y and activation of immune CD4 - and CD8 - T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4-T cell clones. 2000-05 2013-07-03T05:25:55Z 2013-07-03T05:25:55Z Journal Article https://hdl.handle.net/10568/33010 en Limited Access Elsevier Journal of Immunological Methods;239: 85-93 |
| spellingShingle | cattle antigens bovidae cells antibodies theileria parva Ballingall, Keith T. Mwangi, David Miano MacHugh, Niall D. Taracha, E.L.N. Totte, P. McKeever, Declan J. A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells |
| title | A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells |
| title_full | A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells |
| title_fullStr | A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells |
| title_full_unstemmed | A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells |
| title_short | A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells |
| title_sort | highly sensitive non radioactive assay for t cell activation in cattle applications in screening for antigens recognised by cd4 and cd8 t cells |
| topic | cattle antigens bovidae cells antibodies theileria parva |
| url | https://hdl.handle.net/10568/33010 |
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