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Establishment of bovine chromosome region specific high resolution maps using microdissection

An alternative approach for the direct analysis of chromosome regions corresponding to economical traits, based on chromosome microdissection, is described. Large fragment clones isolated with primer pairs designed from chromosome fragment-specific DNA sequences were localized by fluorescence in sit...

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Main Authors: Goldammer, T., Brunner, R.M., Kang'a, S., Hanotte, Olivier H., Schwerin, M.
Format: Journal Article
Language:Inglés
Published: Informa UK Limited 1999
Subjects:
bovidae
chromosomes
genetic maps
technology
dna hybridization
dna
nucleotide sequence
biotechnology
cattle
Online Access:https://hdl.handle.net/10568/30041
Summary:An alternative approach for the direct analysis of chromosome regions corresponding to economical traits, based on chromosome microdissection, is described. Large fragment clones isolated with primer pairs designed from chromosome fragment-specific DNA sequences were localized by fluorescence in situ hybridization (FISH) to the scraped chromosome region of interest.

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