Trypanocidal failure suggested by PCR results in cattle field samples
The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2004
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/29971 |
| _version_ | 1855518246758776832 |
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| author | Gall, Y. Woitag, T. Bauer, B. Sidibe, I. McDermott, John J. Mehlitz, D. Clausen, Peter-Henning |
| author_browse | Bauer, B. Clausen, Peter-Henning Gall, Y. McDermott, John J. Mehlitz, D. Sidibe, I. Woitag, T. |
| author_facet | Gall, Y. Woitag, T. Bauer, B. Sidibe, I. McDermott, John J. Mehlitz, D. Clausen, Peter-Henning |
| author_sort | Gall, Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and 28 days after controlled treatment of 738 cattle from 10 villages in Kenedougou, Burkina Faso with isometamidium chloride and diminazene aceturate. Using three sets of primers, PCR was three-four times more sensitive and better at species identification, than standard microscopic examination. The better sensitivity and species specificity of PCR have important advantages for drug resistance studies in the field. |
| format | Journal Article |
| id | CGSpace29971 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace299712024-03-06T10:16:43Z Trypanocidal failure suggested by PCR results in cattle field samples Gall, Y. Woitag, T. Bauer, B. Sidibe, I. McDermott, John J. Mehlitz, D. Clausen, Peter-Henning phytomonas trypanosomiasis antiprotozoal agents therapy pcr cattle infectious diseases parasitology The aim of this study was to assess, whether polymerase chain reaction (PCR) allows sensitive screening of treatment failure suspicions in areas, where drug resistance against African animal trypanosomosis (AAT) appears to be a problem. PCR was used to detect trypanosome infections prior to, 14 and 28 days after controlled treatment of 738 cattle from 10 villages in Kenedougou, Burkina Faso with isometamidium chloride and diminazene aceturate. Using three sets of primers, PCR was three-four times more sensitive and better at species identification, than standard microscopic examination. The better sensitivity and species specificity of PCR have important advantages for drug resistance studies in the field. 2004-09 2013-06-11T09:25:39Z 2013-06-11T09:25:39Z Journal Article https://hdl.handle.net/10568/29971 en Limited Access Elsevier Acta Tropica;92(1): 7-16 |
| spellingShingle | phytomonas trypanosomiasis antiprotozoal agents therapy pcr cattle infectious diseases parasitology Gall, Y. Woitag, T. Bauer, B. Sidibe, I. McDermott, John J. Mehlitz, D. Clausen, Peter-Henning Trypanocidal failure suggested by PCR results in cattle field samples |
| title | Trypanocidal failure suggested by PCR results in cattle field samples |
| title_full | Trypanocidal failure suggested by PCR results in cattle field samples |
| title_fullStr | Trypanocidal failure suggested by PCR results in cattle field samples |
| title_full_unstemmed | Trypanocidal failure suggested by PCR results in cattle field samples |
| title_short | Trypanocidal failure suggested by PCR results in cattle field samples |
| title_sort | trypanocidal failure suggested by pcr results in cattle field samples |
| topic | phytomonas trypanosomiasis antiprotozoal agents therapy pcr cattle infectious diseases parasitology |
| url | https://hdl.handle.net/10568/29971 |
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