African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro
Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristi...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Association for the Advancement of Science
1977
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29601 |
| Summary: | Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces. |
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