African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro
Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristi...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
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American Association for the Advancement of Science
1977
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29601 |
| _version_ | 1855532655214329856 |
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| author | Hirumi, H. Doyle, J.J. Hirumi, K. |
| author_browse | Doyle, J.J. Hirumi, H. Hirumi, K. |
| author_facet | Hirumi, H. Doyle, J.J. Hirumi, K. |
| author_sort | Hirumi, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces. |
| format | Journal Article |
| id | CGSpace29601 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1977 |
| publishDateRange | 1977 |
| publishDateSort | 1977 |
| publisher | American Association for the Advancement of Science |
| publisherStr | American Association for the Advancement of Science |
| record_format | dspace |
| spelling | CGSpace296012024-05-01T08:18:32Z African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro Hirumi, H. Doyle, J.J. Hirumi, K. trypanosoma brucei animal diseases Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces. 1977-05-27 2013-06-11T09:24:09Z 2013-06-11T09:24:09Z Journal Article https://hdl.handle.net/10568/29601 en Limited Access American Association for the Advancement of Science Science;196: 992-994 |
| spellingShingle | trypanosoma brucei animal diseases Hirumi, H. Doyle, J.J. Hirumi, K. African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro |
| title | African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro |
| title_full | African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro |
| title_fullStr | African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro |
| title_full_unstemmed | African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro |
| title_short | African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro |
| title_sort | african trypanosomes cultivation of animal infective trypanosoma brucei in vitro |
| topic | trypanosoma brucei animal diseases |
| url | https://hdl.handle.net/10568/29601 |
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