Cover Image
QR Code

Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle

Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat sa...

Full description

Main Authors: Majiwa, Phelix A.O., Thatthi, R., Moloo, S.K., Nyeko, J.H.P., Otieno, L.H., Maloo, S.
Format: Journal Article
Language:Inglés
Published: Cambridge University Press 1994
Subjects:
trypanosoma
cattle
parasites
saliva
infection
glossinidae
animal diseases
Online Access:https://hdl.handle.net/10568/29441
Summary:Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes.

Similar Items