Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle
Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat sa...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Cambridge University Press
1994
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/29441 |
| _version_ | 1855531447813668864 |
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| author | Majiwa, Phelix A.O. Thatthi, R. Moloo, S.K. Nyeko, J.H.P. Otieno, L.H. Maloo, S. |
| author_browse | Majiwa, Phelix A.O. Maloo, S. Moloo, S.K. Nyeko, J.H.P. Otieno, L.H. Thatthi, R. |
| author_facet | Majiwa, Phelix A.O. Thatthi, R. Moloo, S.K. Nyeko, J.H.P. Otieno, L.H. Maloo, S. |
| author_sort | Majiwa, Phelix A.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes. |
| format | Journal Article |
| id | CGSpace29441 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1994 |
| publishDateRange | 1994 |
| publishDateSort | 1994 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace294412024-11-15T08:52:46Z Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle Majiwa, Phelix A.O. Thatthi, R. Moloo, S.K. Nyeko, J.H.P. Otieno, L.H. Maloo, S. trypanosoma cattle parasites saliva infection glossinidae animal diseases Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypanosomes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes. 1994-04 2013-06-11T09:23:34Z 2013-06-11T09:23:34Z Journal Article https://hdl.handle.net/10568/29441 en Limited Access Cambridge University Press Parasitology;108: 313-322 |
| spellingShingle | trypanosoma cattle parasites saliva infection glossinidae animal diseases Majiwa, Phelix A.O. Thatthi, R. Moloo, S.K. Nyeko, J.H.P. Otieno, L.H. Maloo, S. Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle |
| title | Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle |
| title_full | Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle |
| title_fullStr | Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle |
| title_full_unstemmed | Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle |
| title_short | Detection of trypanosome infection in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle |
| title_sort | detection of trypanosome infection in the saliva of tsetse flies and buffy coat samples from antigenaemic but aparasitaemic cattle |
| topic | trypanosoma cattle parasites saliva infection glossinidae animal diseases |
| url | https://hdl.handle.net/10568/29441 |
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