Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing seque...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2001
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28253 |
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