Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing seque...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2001
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/28253 |
| _version_ | 1855523140181950464 |
|---|---|
| author | Wambugu, J. Kanguha, E. Hanotte, Olivier H. Davis, S. Taylor, J. Skow, L. Brunner, R. Schwerin, M. Teale, A.J. Iraqi, F.A. |
| author_browse | Brunner, R. Davis, S. Hanotte, Olivier H. Iraqi, F.A. Kanguha, E. Schwerin, M. Skow, L. Taylor, J. Teale, A.J. Wambugu, J. |
| author_facet | Wambugu, J. Kanguha, E. Hanotte, Olivier H. Davis, S. Taylor, J. Skow, L. Brunner, R. Schwerin, M. Teale, A.J. Iraqi, F.A. |
| author_sort | Wambugu, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing sequences homologous with the probe was sequenced and a (GT)9 microsatellite was identified. Further DNA sequencing for the subclones yielded a (TA)10(TTA)5 microsatellite. Primers were designed to amplify these 2 microsatellites in 4 cattle breeds. The PCR product length was polymorphic as shown by denaturing polyacrylamide gel electrophoresis. The microsatellites were designated as ILFI-TNF-I and II for (GT)9 and (TA)10(TTA)5, respectively. |
| format | Journal Article |
| id | CGSpace28253 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2001 |
| publishDateRange | 2001 |
| publishDateSort | 2001 |
| record_format | dspace |
| spelling | CGSpace282532022-01-29T16:04:32Z Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone Wambugu, J. Kanguha, E. Hanotte, Olivier H. Davis, S. Taylor, J. Skow, L. Brunner, R. Schwerin, M. Teale, A.J. Iraqi, F.A. cattle genes genetic polymorphism microsatellites nucleotide sequences tumour necrosis factor Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing sequences homologous with the probe was sequenced and a (GT)9 microsatellite was identified. Further DNA sequencing for the subclones yielded a (TA)10(TTA)5 microsatellite. Primers were designed to amplify these 2 microsatellites in 4 cattle breeds. The PCR product length was polymorphic as shown by denaturing polyacrylamide gel electrophoresis. The microsatellites were designated as ILFI-TNF-I and II for (GT)9 and (TA)10(TTA)5, respectively. 2001 2013-05-06T07:00:15Z 2013-05-06T07:00:15Z Journal Article https://hdl.handle.net/10568/28253 en Limited Access Animal Genetics;32(2): 105-121 |
| spellingShingle | cattle genes genetic polymorphism microsatellites nucleotide sequences tumour necrosis factor Wambugu, J. Kanguha, E. Hanotte, Olivier H. Davis, S. Taylor, J. Skow, L. Brunner, R. Schwerin, M. Teale, A.J. Iraqi, F.A. Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone |
| title | Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone |
| title_full | Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone |
| title_fullStr | Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone |
| title_full_unstemmed | Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone |
| title_short | Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone |
| title_sort | identification of two polymorphic microsatellite repeats on a bovine tnfa containing bac clone |
| topic | cattle genes genetic polymorphism microsatellites nucleotide sequences tumour necrosis factor |
| url | https://hdl.handle.net/10568/28253 |
| work_keys_str_mv | AT wambuguj identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT kanguhae identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT hanotteolivierh identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT daviss identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT taylorj identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT skowl identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT brunnerr identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT schwerinm identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT tealeaj identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone AT iraqifa identificationoftwopolymorphicmicrosatelliterepeatsonabovinetnfacontainingbacclone |