The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters

Six sheep were fed twice a day on a different ratio of sheep pellets and hay (20:80 (diet 1), 40:60 (diet 2) and 80:20 (diet 3)) in a replicated Latin square design to study the effect of the host diet on the bacterial concentration of ruminal liquor and in vitro gas production degradability paramet...

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Main Authors: Nagadi, S., Herrero, Mario, Jessop, N.S.
Format: Journal Article
Language:Inglés
Published: 2000
Subjects:
Online Access:https://hdl.handle.net/10568/2023
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author Nagadi, S.
Herrero, Mario
Jessop, N.S.
author_browse Herrero, Mario
Jessop, N.S.
Nagadi, S.
author_facet Nagadi, S.
Herrero, Mario
Jessop, N.S.
author_sort Nagadi, S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Six sheep were fed twice a day on a different ratio of sheep pellets and hay (20:80 (diet 1), 40:60 (diet 2) and 80:20 (diet 3)) in a replicated Latin square design to study the effect of the host diet on the bacterial concentration of ruminal liquor and in vitro gas production degradability parameters of cellulose, glucose and hay. Bacterial DM, bacterial absorbance and the volume of gas produced in the absence of substrate increased as the ratio of sheep pellet to hay increased. The gas production degradability parameters obtained from fitting data to the model Gas = B(1 - exp-C(t-Lag)) were also affected by changing the ratio of sheep pellets to hay in the diet of donor animals. For each substrate, incubation with ruminal fluid taken from sheep fed on diet 2 or 3 gave higher (P < 0.05) asymptotic values 'B' (except for hay), rates C' of gas production and lower Lag times (cellulose and hay only) than when incubated in the ruminal fluid taken from sheep fed on diet 1. The digestibility of NDF from cellulose and hay was not affected by diet. Bacterial DM was strongly related to the absorbance of ruminal fluid and the volume of gas produced in the absence of substrate (R2 = 0.99, P < 0.001). Results suggest that changing the ratio of concentrate to hay reduces the initial bacterial concentration and affects the gas production degradability parameters but the estimation of bacterial DM either from bacterial absorbance or volume of gas produced without substrate was not affected by changing the diet of donor animal.
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spelling CGSpace20232024-01-22T09:44:16Z The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters Nagadi, S. Herrero, Mario Jessop, N.S. biodegradability rumen digestion digestibility Six sheep were fed twice a day on a different ratio of sheep pellets and hay (20:80 (diet 1), 40:60 (diet 2) and 80:20 (diet 3)) in a replicated Latin square design to study the effect of the host diet on the bacterial concentration of ruminal liquor and in vitro gas production degradability parameters of cellulose, glucose and hay. Bacterial DM, bacterial absorbance and the volume of gas produced in the absence of substrate increased as the ratio of sheep pellet to hay increased. The gas production degradability parameters obtained from fitting data to the model Gas = B(1 - exp-C(t-Lag)) were also affected by changing the ratio of sheep pellets to hay in the diet of donor animals. For each substrate, incubation with ruminal fluid taken from sheep fed on diet 2 or 3 gave higher (P < 0.05) asymptotic values 'B' (except for hay), rates C' of gas production and lower Lag times (cellulose and hay only) than when incubated in the ruminal fluid taken from sheep fed on diet 1. The digestibility of NDF from cellulose and hay was not affected by diet. Bacterial DM was strongly related to the absorbance of ruminal fluid and the volume of gas produced in the absence of substrate (R2 = 0.99, P < 0.001). Results suggest that changing the ratio of concentrate to hay reduces the initial bacterial concentration and affects the gas production degradability parameters but the estimation of bacterial DM either from bacterial absorbance or volume of gas produced without substrate was not affected by changing the diet of donor animal. 2000 2010-07-05T19:49:31Z 2010-07-05T19:49:31Z Journal Article https://hdl.handle.net/10568/2023 en Limited Access Nagadi, S; Herrero, M.; Jessop, N.S. 2000 .The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters. Animal Feed Science and Technology. 87: 231-239
spellingShingle biodegradability
rumen digestion
digestibility
Nagadi, S.
Herrero, Mario
Jessop, N.S.
The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
title The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
title_full The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
title_fullStr The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
title_full_unstemmed The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
title_short The influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
title_sort influence of diet of the donor animal on the initial bacterial concentration of ruminal fluid and in vitro gas production degradability parameters
topic biodegradability
rumen digestion
digestibility
url https://hdl.handle.net/10568/2023
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