Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis
Peanut (Arachis hypogaea L.) is a globally significant oil and economic crop, serving as a primary source of edible oil and protein. Aflatoxin contamination is a main risk factor for peanut food safety and industry development worldwide. The most cost-economic and effective control strategy entails...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2025
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/180014 |
| _version_ | 1855516730179190784 |
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| author | Huai, Dongxin Huang, Li Xue, Xiaomeng Yu, Bolun Ding, Yingbin Jin, Gaorui Liu, Hao Pandey, Manish K. Sudini, Hari Kishan Luo, Huaiyong Zhou, Xiaojing Liu, Nian Chen, Weigang Yan, Liying Chen, Yuning Wang, Xin Wang, Qianqian Kang, Yanping Wang, Zhihui Chen, Xiaoping Jiang, Huifang Lei, Yong Liao, Boshou |
| author_browse | Chen, Weigang Chen, Xiaoping Chen, Yuning Ding, Yingbin Huai, Dongxin Huang, Li Jiang, Huifang Jin, Gaorui Kang, Yanping Lei, Yong Liao, Boshou Liu, Hao Liu, Nian Luo, Huaiyong Pandey, Manish K. Sudini, Hari Kishan Wang, Qianqian Wang, Xin Wang, Zhihui Xue, Xiaomeng Yan, Liying Yu, Bolun Zhou, Xiaojing |
| author_facet | Huai, Dongxin Huang, Li Xue, Xiaomeng Yu, Bolun Ding, Yingbin Jin, Gaorui Liu, Hao Pandey, Manish K. Sudini, Hari Kishan Luo, Huaiyong Zhou, Xiaojing Liu, Nian Chen, Weigang Yan, Liying Chen, Yuning Wang, Xin Wang, Qianqian Kang, Yanping Wang, Zhihui Chen, Xiaoping Jiang, Huifang Lei, Yong Liao, Boshou |
| author_sort | Huai, Dongxin |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Peanut (Arachis hypogaea L.) is a globally significant oil and economic crop, serving as a primary source of edible oil and protein. Aflatoxin contamination is a main risk factor for peanut food safety and industry development worldwide. The most cost-economic and effective control strategy entails the exploration and utilization of natural resistance in peanut, alongside the development of resistant varieties. However, the underlying mechanism of resistance to aflatoxin production (AP) in peanuts remains elusive. In this study, a RIL population derived from a cross between Zhonghua 10 (susceptible) and ICG 12625 (resistant), was used to identify quantitative trait loci (QTLs) for AP resistance. Overall, seven QTLs associated with AP resistance were mapped on five chromosomes, explaining 6.83–17.86% of phenotypic variance (PVE). Notably, only two major QTLs, namely qAftA07and qAftB06.2, were consistently detected across different environments with 6.83–16.52% PVE. To predict the candidate genes for AP resistance in qAftA07and qAftB06.2, the transcriptome analysis of seeds from parental lines inoculated with Aspergillus flavus were conducted. A total of 175 and 238 candidate genes were respectively identified in qAftA07 and qAftB06.2, encompassing genes with non-synonymous genomic variations as well as differentially expressed genes. Combined with the weighted gene co-expression network analysis, 10 and 11 genes in qAftA07 and qAftB06.2 were characterized showing a high correlation with aflatoxin content, thereby representing the most promising candidate genes within these two QTLs. These results provide valuable insights for future map-based cloning studies targeting candidate genes associated with AP resistance in peanut. |
| format | Journal Article |
| id | CGSpace180014 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1800142026-01-17T02:02:06Z Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis Huai, Dongxin Huang, Li Xue, Xiaomeng Yu, Bolun Ding, Yingbin Jin, Gaorui Liu, Hao Pandey, Manish K. Sudini, Hari Kishan Luo, Huaiyong Zhou, Xiaojing Liu, Nian Chen, Weigang Yan, Liying Chen, Yuning Wang, Xin Wang, Qianqian Kang, Yanping Wang, Zhihui Chen, Xiaoping Jiang, Huifang Lei, Yong Liao, Boshou aflatoxins arachis hypogaea food safety resistant varieties aspergillus flavus Peanut (Arachis hypogaea L.) is a globally significant oil and economic crop, serving as a primary source of edible oil and protein. Aflatoxin contamination is a main risk factor for peanut food safety and industry development worldwide. The most cost-economic and effective control strategy entails the exploration and utilization of natural resistance in peanut, alongside the development of resistant varieties. However, the underlying mechanism of resistance to aflatoxin production (AP) in peanuts remains elusive. In this study, a RIL population derived from a cross between Zhonghua 10 (susceptible) and ICG 12625 (resistant), was used to identify quantitative trait loci (QTLs) for AP resistance. Overall, seven QTLs associated with AP resistance were mapped on five chromosomes, explaining 6.83–17.86% of phenotypic variance (PVE). Notably, only two major QTLs, namely qAftA07and qAftB06.2, were consistently detected across different environments with 6.83–16.52% PVE. To predict the candidate genes for AP resistance in qAftA07and qAftB06.2, the transcriptome analysis of seeds from parental lines inoculated with Aspergillus flavus were conducted. A total of 175 and 238 candidate genes were respectively identified in qAftA07 and qAftB06.2, encompassing genes with non-synonymous genomic variations as well as differentially expressed genes. Combined with the weighted gene co-expression network analysis, 10 and 11 genes in qAftA07 and qAftB06.2 were characterized showing a high correlation with aflatoxin content, thereby representing the most promising candidate genes within these two QTLs. These results provide valuable insights for future map-based cloning studies targeting candidate genes associated with AP resistance in peanut. 2025-03-13 2026-01-16T18:11:02Z 2026-01-16T18:11:02Z Journal Article https://hdl.handle.net/10568/180014 en Open Access application/pdf Springer Huai, Dongxin; Huang, Li; Xue, Xiaomeng; Yu, Bolun; Ding, Yingbin; Jin, Gaorui; Liu, Hao; Pandey, Manish K.; Sudini, Hari Kishan; Luo, Huaiyong; Zhou, Xiaojing; Liu, Nian; Chen, Weigang; Yan, Liying; Chen, Yuning; Wang, Xin; Wang, Qianqian; Kang, Yanping; Wang, Zhihui; Chen, Xiaoping; Jiang, Huifang; Lei, Yong; & Liao, Boshou. 2025. Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis. Theor Appl Genet 138, 71 (2025). https://doi.org/10.1007/s00122-025-04822-1 |
| spellingShingle | aflatoxins arachis hypogaea food safety resistant varieties aspergillus flavus Huai, Dongxin Huang, Li Xue, Xiaomeng Yu, Bolun Ding, Yingbin Jin, Gaorui Liu, Hao Pandey, Manish K. Sudini, Hari Kishan Luo, Huaiyong Zhou, Xiaojing Liu, Nian Chen, Weigang Yan, Liying Chen, Yuning Wang, Xin Wang, Qianqian Kang, Yanping Wang, Zhihui Chen, Xiaoping Jiang, Huifang Lei, Yong Liao, Boshou Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| title | Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| title_full | Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| title_fullStr | Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| title_full_unstemmed | Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| title_short | Identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| title_sort | identification of candidate genes associated with resistance to aflatoxin production in peanut through genetic mapping and transcriptome analysis |
| topic | aflatoxins arachis hypogaea food safety resistant varieties aspergillus flavus |
| url | https://hdl.handle.net/10568/180014 |
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