PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder
This paper presents the design, construction, operation, and evaluation of PowderBot, a purpose-built, open-source, low-cost machine (∼US$ 5000) that automates DNA extraction from ungerminated seeds. The device drills into the seed cotyledon, where the genetic information of the prospective plant is...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier BV
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/179338 |
| _version_ | 1855527026905055232 |
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| author | Díaz Rodríguez, Harold Andrés Macea, Eliana Escobar, Roosevelt Beebe, Stephen Tohme, Joseph Raatz, Bodo |
| author_browse | Beebe, Stephen Díaz Rodríguez, Harold Andrés Escobar, Roosevelt Macea, Eliana Raatz, Bodo Tohme, Joseph |
| author_facet | Díaz Rodríguez, Harold Andrés Macea, Eliana Escobar, Roosevelt Beebe, Stephen Tohme, Joseph Raatz, Bodo |
| author_sort | Díaz Rodríguez, Harold Andrés |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | This paper presents the design, construction, operation, and evaluation of PowderBot, a purpose-built, open-source, low-cost machine (∼US$ 5000) that automates DNA extraction from ungerminated seeds. The device drills into the seed cotyledon, where the genetic information of the prospective plant is stored. It then transfers the pure, powdered samples directly to well-plates for analysis. This reduces time and other research resources and can accelerate crop varietal improvement, ultimately contributing to more efficient and successful crop breeding programs. At CIAT́s campus, we have validated the method for obtaining seed-tissue material from common beans for DNA extraction and subsequently genotyping agronomically interesting lines, using the bc-3 molecular marker. Three genotyping trials were carried out using this method, which generated consistent results, regardless of the number of perforations made to the seed. This leads us to infer that the method works effectively and can be applied for marker assisted selection (MAS) in bean and other crop breeding programs. Finally, germination and vigor tests indicated the sampling process did not significantly compromise perforated seed germination rate, physiological quality or viability. |
| format | Journal Article |
| id | CGSpace179338 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier BV |
| publisherStr | Elsevier BV |
| record_format | dspace |
| spelling | CGSpace1793382026-01-01T02:08:14Z PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder Díaz Rodríguez, Harold Andrés Macea, Eliana Escobar, Roosevelt Beebe, Stephen Tohme, Joseph Raatz, Bodo beans plant breeding genotyping dna technology analysis This paper presents the design, construction, operation, and evaluation of PowderBot, a purpose-built, open-source, low-cost machine (∼US$ 5000) that automates DNA extraction from ungerminated seeds. The device drills into the seed cotyledon, where the genetic information of the prospective plant is stored. It then transfers the pure, powdered samples directly to well-plates for analysis. This reduces time and other research resources and can accelerate crop varietal improvement, ultimately contributing to more efficient and successful crop breeding programs. At CIAT́s campus, we have validated the method for obtaining seed-tissue material from common beans for DNA extraction and subsequently genotyping agronomically interesting lines, using the bc-3 molecular marker. Three genotyping trials were carried out using this method, which generated consistent results, regardless of the number of perforations made to the seed. This leads us to infer that the method works effectively and can be applied for marker assisted selection (MAS) in bean and other crop breeding programs. Finally, germination and vigor tests indicated the sampling process did not significantly compromise perforated seed germination rate, physiological quality or viability. 2025-12 2025-12-31T10:11:32Z 2025-12-31T10:11:32Z Journal Article https://hdl.handle.net/10568/179338 en Open Access application/pdf Elsevier BV Díaz Rodríguez, H.A.; Macea, E.; Escobar, R.; Beebe, S.; Tohme, J.; Raatz, B. (2025) PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder. HardwareX 24: e00706. ISSN: 2468-0672 |
| spellingShingle | beans plant breeding genotyping dna technology analysis Díaz Rodríguez, Harold Andrés Macea, Eliana Escobar, Roosevelt Beebe, Stephen Tohme, Joseph Raatz, Bodo PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder |
| title | PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder |
| title_full | PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder |
| title_fullStr | PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder |
| title_full_unstemmed | PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder |
| title_short | PowderBot: An automated device for decision-making in crop breeding programs based on DNA extraction from seed powder |
| title_sort | powderbot an automated device for decision making in crop breeding programs based on dna extraction from seed powder |
| topic | beans plant breeding genotyping dna technology analysis |
| url | https://hdl.handle.net/10568/179338 |
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