Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia
<i>Campylobacter enteritis</i> is the most common bacterial foodborne disease in humans. Long-term use of antibiotics in chicken production may result in antimicrobial resistance in <i>Campylobacter</i> strains. Information on the antimicrobial resistance profile of <i>Campylobacter</i> species amon...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Society of Tropical Medicine and Hygiene
2025
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/175335 |
| _version_ | 1855514583365582848 |
|---|---|
| author | Worku, M. Tessema, B. Ferede, G. Ochieng, Linnet Leliso, S.A. Mutua, Florence Moodley, Arshnee Gelaw, B. Grace, Delia |
| author_browse | Ferede, G. Gelaw, B. Grace, Delia Leliso, S.A. Moodley, Arshnee Mutua, Florence Ochieng, Linnet Tessema, B. Worku, M. |
| author_facet | Worku, M. Tessema, B. Ferede, G. Ochieng, Linnet Leliso, S.A. Mutua, Florence Moodley, Arshnee Gelaw, B. Grace, Delia |
| author_sort | Worku, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | <i>Campylobacter enteritis</i> is the most common bacterial foodborne disease in humans. Long-term use of antibiotics in chicken production may result in antimicrobial resistance in <i>Campylobacter</i> strains. Information on the antimicrobial resistance profile of <i>Campylobacter</i> species among free-range chickens in Ethiopia is scarce. Hence, this study aimed to determine the prevalence and antimicrobial resistance of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> among free-range chickens in Amhara National Regional state, northwest Ethiopia from November 1, 2022 to April 30, 2023. Cloacal swabs were collected from free-range backyard chickens, directly inoculated onto modified charcoal cefoperazone deoxycholate agar, and incubated at reduced O<sub>2</sub> concentration at 42°C for 48 hours. Suspected colonies were confirmed at the species level using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The associated factors were analyzed using the Fisher exact test. A P <0.05 at 95% CI was considered statistically significant. Among the 286 cloacal samples, 15.0% (n = 43/286; CI: 10.2–19.5) were positive for <i>Campylobacter</i> species. <i>C. jejuni</i> (60.5%) was more frequent than <i>C. coli</i> (39.5%). Of the total isolates, 62.8% (n = 27/43), 51.2% (n = 22/43), and 16.3% (n = 7/43) of the <i>Campylobacter</i> species were resistant to tetracycline, ciprofloxacin, and erythromycin, respectively. Of the total <i>Campylobacter</i> species isolates, 9.3% (n = 4/43) were multidrug resistant. <i>Campylobacter</i> species resistance to tetracycline and ciprofloxacin was high in general among backyard chickens. Multidrug-resistant <i>Campylobacter</i> species were also identified, and they require special attention to prevent the potential dissemination of the strains to humans in the community. |
| format | Journal Article |
| id | CGSpace175335 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | American Society of Tropical Medicine and Hygiene |
| publisherStr | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | CGSpace1753352025-12-08T10:06:44Z Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia Worku, M. Tessema, B. Ferede, G. Ochieng, Linnet Leliso, S.A. Mutua, Florence Moodley, Arshnee Gelaw, B. Grace, Delia antimicrobial resistance chickens poultry <i>Campylobacter enteritis</i> is the most common bacterial foodborne disease in humans. Long-term use of antibiotics in chicken production may result in antimicrobial resistance in <i>Campylobacter</i> strains. Information on the antimicrobial resistance profile of <i>Campylobacter</i> species among free-range chickens in Ethiopia is scarce. Hence, this study aimed to determine the prevalence and antimicrobial resistance of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> among free-range chickens in Amhara National Regional state, northwest Ethiopia from November 1, 2022 to April 30, 2023. Cloacal swabs were collected from free-range backyard chickens, directly inoculated onto modified charcoal cefoperazone deoxycholate agar, and incubated at reduced O<sub>2</sub> concentration at 42°C for 48 hours. Suspected colonies were confirmed at the species level using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The associated factors were analyzed using the Fisher exact test. A P <0.05 at 95% CI was considered statistically significant. Among the 286 cloacal samples, 15.0% (n = 43/286; CI: 10.2–19.5) were positive for <i>Campylobacter</i> species. <i>C. jejuni</i> (60.5%) was more frequent than <i>C. coli</i> (39.5%). Of the total isolates, 62.8% (n = 27/43), 51.2% (n = 22/43), and 16.3% (n = 7/43) of the <i>Campylobacter</i> species were resistant to tetracycline, ciprofloxacin, and erythromycin, respectively. Of the total <i>Campylobacter</i> species isolates, 9.3% (n = 4/43) were multidrug resistant. <i>Campylobacter</i> species resistance to tetracycline and ciprofloxacin was high in general among backyard chickens. Multidrug-resistant <i>Campylobacter</i> species were also identified, and they require special attention to prevent the potential dissemination of the strains to humans in the community. 2025-09-03 2025-06-26T10:17:28Z 2025-06-26T10:17:28Z Journal Article https://hdl.handle.net/10568/175335 en Open Access American Society of Tropical Medicine and Hygiene Worku, M., Tessema, B., Ferede, G., Ochieng, L., Leliso, S.A., Mutua, F., Moodley, A., Gelaw, B. and Grace, D. 2025. Prevalence and antimicrobial-resistant <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> in free-range chickens in northwest Ethiopia. American Journal of Tropical Medicine and Hygiene 113(3): 694–700. |
| spellingShingle | antimicrobial resistance chickens poultry Worku, M. Tessema, B. Ferede, G. Ochieng, Linnet Leliso, S.A. Mutua, Florence Moodley, Arshnee Gelaw, B. Grace, Delia Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia |
| title | Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia |
| title_full | Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia |
| title_fullStr | Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia |
| title_full_unstemmed | Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia |
| title_short | Prevalence and antimicrobial-resistant Campylobacter jejuni and Campylobacter coli in free-range chickens in northwest Ethiopia |
| title_sort | prevalence and antimicrobial resistant campylobacter jejuni and campylobacter coli in free range chickens in northwest ethiopia |
| topic | antimicrobial resistance chickens poultry |
| url | https://hdl.handle.net/10568/175335 |
| work_keys_str_mv | AT workum prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT tessemab prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT feredeg prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT ochienglinnet prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT lelisosa prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT mutuaflorence prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT moodleyarshnee prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT gelawb prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia AT gracedelia prevalenceandantimicrobialresistantcampylobacterjejuniandcampylobactercoliinfreerangechickensinnorthwestethiopia |