Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro
The aim of this study was to evaluate the effect of Insulin-Transferrin-Selenium (ITS) supplementation in embryo culture on the blastocyst rate and embryo quality. The bovine zygotes obtained by in vitro maturation (MIV) and fertilization (IVF) were transferred to Evolve medium in 4 groups (T1: With...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Español |
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Universidad Nacional Mayor de San Marcos, Vicerectorado de Investigacion
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/173713 |
| _version_ | 1855532791522918400 |
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| author | Acosta Lobo, Mariano Eliecer Robledo Salgado, Pablo Andres Londono Franco, Luis Fernando Torres Osorio, Viviana Andrea Vasquez Araque, Neil A. |
| author_browse | Acosta Lobo, Mariano Eliecer Londono Franco, Luis Fernando Robledo Salgado, Pablo Andres Torres Osorio, Viviana Andrea Vasquez Araque, Neil A. |
| author_facet | Acosta Lobo, Mariano Eliecer Robledo Salgado, Pablo Andres Londono Franco, Luis Fernando Torres Osorio, Viviana Andrea Vasquez Araque, Neil A. |
| author_sort | Acosta Lobo, Mariano Eliecer |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The aim of this study was to evaluate the effect of Insulin-Transferrin-Selenium (ITS) supplementation in embryo culture on the blastocyst rate and embryo quality. The bovine zygotes obtained by in vitro maturation (MIV) and fertilization (IVF) were transferred to Evolve medium in 4 groups (T1: Without ITS supplement; T2: ITS the first 48 h of culture (CIV1): T3: ITS after day 2 of culture (CIV2), T4: ITS during all culture time (CIV1 + CIV2). All procedures were performed at 38.5 °C, 5% CO2 and 90% relative humidity. The cleavage and blastocyst rates were determined at 48 h and day 8 of culture. The total cell number and the production of reactive oxygen species (ROS) in the expanded blastocysts were determined by staining the nucleus with Hoechst and the oxidation of the fluorescent probe dichlorodihydrofluorescein diacetate (H2DCFDA). The T4 cultured embryos presented the highest rate of blastocysts (32.6%) and total cell number (145.6) compared to the control group without STI (26.2% and 98.6, respectively; p<0.05). Likewise, the production of ROS in T4 blastocysts was lower (p<0.05) than that obtained in the other study groups. The results show that the addition of the ITS supplement throughout the embryo culture time has beneficial effects on the percentage of blastocysts and embryo quality, assessed by the number of total cells and the production of ROS. |
| format | Journal Article |
| id | CGSpace173713 |
| institution | CGIAR Consortium |
| language | Español |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Universidad Nacional Mayor de San Marcos, Vicerectorado de Investigacion |
| publisherStr | Universidad Nacional Mayor de San Marcos, Vicerectorado de Investigacion |
| record_format | dspace |
| spelling | CGSpace1737132025-11-11T19:00:06Z Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro Acosta Lobo, Mariano Eliecer Robledo Salgado, Pablo Andres Londono Franco, Luis Fernando Torres Osorio, Viviana Andrea Vasquez Araque, Neil A. sodium insulin oocysts The aim of this study was to evaluate the effect of Insulin-Transferrin-Selenium (ITS) supplementation in embryo culture on the blastocyst rate and embryo quality. The bovine zygotes obtained by in vitro maturation (MIV) and fertilization (IVF) were transferred to Evolve medium in 4 groups (T1: Without ITS supplement; T2: ITS the first 48 h of culture (CIV1): T3: ITS after day 2 of culture (CIV2), T4: ITS during all culture time (CIV1 + CIV2). All procedures were performed at 38.5 °C, 5% CO2 and 90% relative humidity. The cleavage and blastocyst rates were determined at 48 h and day 8 of culture. The total cell number and the production of reactive oxygen species (ROS) in the expanded blastocysts were determined by staining the nucleus with Hoechst and the oxidation of the fluorescent probe dichlorodihydrofluorescein diacetate (H2DCFDA). The T4 cultured embryos presented the highest rate of blastocysts (32.6%) and total cell number (145.6) compared to the control group without STI (26.2% and 98.6, respectively; p<0.05). Likewise, the production of ROS in T4 blastocysts was lower (p<0.05) than that obtained in the other study groups. The results show that the addition of the ITS supplement throughout the embryo culture time has beneficial effects on the percentage of blastocysts and embryo quality, assessed by the number of total cells and the production of ROS. 2024-02-29 2025-03-19T10:38:23Z 2025-03-19T10:38:23Z Journal Article https://hdl.handle.net/10568/173713 es Open Access application/pdf Universidad Nacional Mayor de San Marcos, Vicerectorado de Investigacion Acosta Lobo, M.E.; Robledo Salgado, P.A.; Londono Franco, L.F.; Torres Osorio, V.A.; Vasquez Araque, N.A.. (2024) Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro. Revista de Investigaciones Veterinarias del Perú 35(1): e25348. ISSN: 1609-9117 |
| spellingShingle | sodium insulin oocysts Acosta Lobo, Mariano Eliecer Robledo Salgado, Pablo Andres Londono Franco, Luis Fernando Torres Osorio, Viviana Andrea Vasquez Araque, Neil A. Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| title | Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| title_full | Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| title_fullStr | Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| title_full_unstemmed | Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| title_short | Efecto del suplemento Insulina-Transferrina-Selenio (ITS) sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| title_sort | efecto del suplemento insulina transferrina selenio its sobre la tasa de desarrollo y calidad de embriones bovinos in vitro |
| topic | sodium insulin oocysts |
| url | https://hdl.handle.net/10568/173713 |
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