Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection

Introduction: Annually, approximately 174 million people globally are affected by <i>Trichomonas vaginalis</i> (<i>T. vaginalis</i>) infection. Half of these infections occur in resource-limited regions. Untreated <i>T. vaginalis</i> infections are associated with complications such as pelvic inflam...

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Autores principales: Shiluli, C., Kamath, S., Kanoi, B.N., Kimani, R., Oduor, Bernard, Abkallo, Hussein M., Maina, M., Waweru, H., Kamita, M., Pamme, N., Dupaty, J., Klapperich, C.M., Lolabattu, S.R., Gitaka, J.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2025
Materias:
Acceso en línea:https://hdl.handle.net/10568/172975
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author Shiluli, C.
Kamath, S.
Kanoi, B.N.
Kimani, R.
Oduor, Bernard
Abkallo, Hussein M.
Maina, M.
Waweru, H.
Kamita, M.
Pamme, N.
Dupaty, J.
Klapperich, C.M.
Lolabattu, S.R.
Gitaka, J.
author_browse Abkallo, Hussein M.
Dupaty, J.
Gitaka, J.
Kamath, S.
Kamita, M.
Kanoi, B.N.
Kimani, R.
Klapperich, C.M.
Lolabattu, S.R.
Maina, M.
Oduor, Bernard
Pamme, N.
Shiluli, C.
Waweru, H.
author_facet Shiluli, C.
Kamath, S.
Kanoi, B.N.
Kimani, R.
Oduor, Bernard
Abkallo, Hussein M.
Maina, M.
Waweru, H.
Kamita, M.
Pamme, N.
Dupaty, J.
Klapperich, C.M.
Lolabattu, S.R.
Gitaka, J.
author_sort Shiluli, C.
collection Repository of Agricultural Research Outputs (CGSpace)
description Introduction: Annually, approximately 174 million people globally are affected by <i>Trichomonas vaginalis</i> (<i>T. vaginalis</i>) infection. Half of these infections occur in resource-limited regions. Untreated <i>T. vaginalis</i> infections are associated with complications such as pelvic inflammatory disease and adverse pregnancy outcomes mostly seen in women. In resource-limited regions, the World Health Organization (WHO) advocates for syndromic case management. However, this can lead to unnecessary treatment. Accurate diagnosis of <i>T. vaginalis</i> is required for effective and prompt treatment. Molecular tests such as Polymerase Chain Reaction (PCR) have the advantage of having a short turn-around time and allow the use of non-invasive specimens such as urine and vaginal swabs. However, these diagnostic techniques have numerous disadvantages such as high infrastructure costs, false negative and positive results, and interstrain variation among others. This study aimed to evaluate the use of identic
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spelling CGSpace1729752025-10-26T12:51:12Z Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection Shiluli, C. Kamath, S. Kanoi, B.N. Kimani, R. Oduor, Bernard Abkallo, Hussein M. Maina, M. Waweru, H. Kamita, M. Pamme, N. Dupaty, J. Klapperich, C.M. Lolabattu, S.R. Gitaka, J. diagnosis health Introduction: Annually, approximately 174 million people globally are affected by <i>Trichomonas vaginalis</i> (<i>T. vaginalis</i>) infection. Half of these infections occur in resource-limited regions. Untreated <i>T. vaginalis</i> infections are associated with complications such as pelvic inflammatory disease and adverse pregnancy outcomes mostly seen in women. In resource-limited regions, the World Health Organization (WHO) advocates for syndromic case management. However, this can lead to unnecessary treatment. Accurate diagnosis of <i>T. vaginalis</i> is required for effective and prompt treatment. Molecular tests such as Polymerase Chain Reaction (PCR) have the advantage of having a short turn-around time and allow the use of non-invasive specimens such as urine and vaginal swabs. However, these diagnostic techniques have numerous disadvantages such as high infrastructure costs, false negative and positive results, and interstrain variation among others. This study aimed to evaluate the use of identic 2025-02-07 2025-02-12T05:59:35Z 2025-02-12T05:59:35Z Journal Article https://hdl.handle.net/10568/172975 en Open Access Shiluli, C., Kamath, S., Kanoi, B.N., Kimani, R., Oduor, B., Abkallo, H.M., Maina, M., Waweru, H., Kamita, M., Pamme, N., Dupaty, J., Klapperich, C.M., Lolabattu, S.R. and Gitaka, J. 2025. Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of <i>Trichomonas vaginalis</i> infection. PLOS ONE 20(2): e0317958.
spellingShingle diagnosis
health
Shiluli, C.
Kamath, S.
Kanoi, B.N.
Kimani, R.
Oduor, Bernard
Abkallo, Hussein M.
Maina, M.
Waweru, H.
Kamita, M.
Pamme, N.
Dupaty, J.
Klapperich, C.M.
Lolabattu, S.R.
Gitaka, J.
Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection
title Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection
title_full Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection
title_fullStr Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection
title_full_unstemmed Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection
title_short Highly sensitive molecular assay based on Identical Multi-Repeat Sequence (IMRS) algorithm for the detection of Trichomonas vaginalis infection
title_sort highly sensitive molecular assay based on identical multi repeat sequence imrs algorithm for the detection of trichomonas vaginalis infection
topic diagnosis
health
url https://hdl.handle.net/10568/172975
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