| Summary: | Introduction: Annually, approximately 174 million people globally are affected by <i>Trichomonas vaginalis</i> (<i>T. vaginalis</i>) infection. Half of these infections occur in resource-limited regions. Untreated <i>T. vaginalis</i> infections are associated with complications such as pelvic inflammatory disease and adverse pregnancy outcomes mostly seen in women. In resource-limited regions, the World Health Organization (WHO) advocates for syndromic case management. However, this can lead to unnecessary treatment. Accurate diagnosis of <i>T. vaginalis</i> is required for effective and prompt treatment. Molecular tests such as Polymerase Chain Reaction (PCR) have the advantage of having a short turn-around time and allow the use of non-invasive specimens such as urine and vaginal swabs. However, these diagnostic techniques have numerous disadvantages such as high infrastructure costs, false negative and positive results, and interstrain variation among others. This study aimed to evaluate the use of identic
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