Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway
Since the creation of "Golden Rice", biofortification of plant-derived foods is a promising strategy for the alleviation of nutritional deficiencies. Potato is the most important staple food for mankind after the cereals rice, wheat and maize, and is extremely poor in provitamin A carotenoids.We tra...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Public Library of Science
2007
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| Acceso en línea: | https://hdl.handle.net/10568/171920 |
| _version_ | 1855537997637746688 |
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| author | Diretto, Gianfranco Al-Babili, Salim Tavazza, Raffaela Papacchioli, Velia Beyer, Peter Giuliano, Giovanni |
| author_browse | Al-Babili, Salim Beyer, Peter Diretto, Gianfranco Giuliano, Giovanni Papacchioli, Velia Tavazza, Raffaela |
| author_facet | Diretto, Gianfranco Al-Babili, Salim Tavazza, Raffaela Papacchioli, Velia Beyer, Peter Giuliano, Giovanni |
| author_sort | Diretto, Gianfranco |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Since the creation of "Golden Rice", biofortification of plant-derived foods is a promising strategy for the alleviation of nutritional deficiencies. Potato is the most important staple food for mankind after the cereals rice, wheat and maize, and is extremely poor in provitamin A carotenoids.We transformed potato with a mini-pathway of bacterial origin, driving the synthesis of beta-carotene (Provitamin A) from geranylgeranyl diphosphate. Three genes, encoding phytoene synthase (CrtB), phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) from Erwinia, under tuber-specific or constitutive promoter control, were used. 86 independent transgenic lines, containing six different promoter/gene combinations, were produced and analyzed. Extensive regulatory effects on the expression of endogenous genes for carotenoid biosynthesis are observed in transgenic lines. Constitutive expression of the CrtY and/or CrtI genes interferes with the establishment of transgenosis and with the accumulation of leaf carotenoids. Expression of all three genes, under tuber-specific promoter control, results in tubers with a deep yellow ("golden") phenotype without any adverse leaf phenotypes. In these tubers, carotenoids increase approx. 20-fold, to 114 mcg/g dry weight and beta-carotene 3600-fold, to 47 mcg/g dry weight.This is the highest carotenoid and beta-carotene content reported for biofortified potato as well as for any of the four major staple foods (the next best event being "Golden Rice 2", with 31 mcg/g dry weight beta-carotene). Assuming a beta-carotene to retinol conversion of 6ratio1, this is sufficient to provide 50% of the Recommended Daily Allowance of Vitamin A with 250 gms (fresh weight) of "golden" potatoes. |
| format | Journal Article |
| id | CGSpace171920 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace1719202025-09-25T13:01:45Z Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway Diretto, Gianfranco Al-Babili, Salim Tavazza, Raffaela Papacchioli, Velia Beyer, Peter Giuliano, Giovanni potatoes carotenoids retinol plant breeding Since the creation of "Golden Rice", biofortification of plant-derived foods is a promising strategy for the alleviation of nutritional deficiencies. Potato is the most important staple food for mankind after the cereals rice, wheat and maize, and is extremely poor in provitamin A carotenoids.We transformed potato with a mini-pathway of bacterial origin, driving the synthesis of beta-carotene (Provitamin A) from geranylgeranyl diphosphate. Three genes, encoding phytoene synthase (CrtB), phytoene desaturase (CrtI) and lycopene beta-cyclase (CrtY) from Erwinia, under tuber-specific or constitutive promoter control, were used. 86 independent transgenic lines, containing six different promoter/gene combinations, were produced and analyzed. Extensive regulatory effects on the expression of endogenous genes for carotenoid biosynthesis are observed in transgenic lines. Constitutive expression of the CrtY and/or CrtI genes interferes with the establishment of transgenosis and with the accumulation of leaf carotenoids. Expression of all three genes, under tuber-specific promoter control, results in tubers with a deep yellow ("golden") phenotype without any adverse leaf phenotypes. In these tubers, carotenoids increase approx. 20-fold, to 114 mcg/g dry weight and beta-carotene 3600-fold, to 47 mcg/g dry weight.This is the highest carotenoid and beta-carotene content reported for biofortified potato as well as for any of the four major staple foods (the next best event being "Golden Rice 2", with 31 mcg/g dry weight beta-carotene). Assuming a beta-carotene to retinol conversion of 6ratio1, this is sufficient to provide 50% of the Recommended Daily Allowance of Vitamin A with 250 gms (fresh weight) of "golden" potatoes. 2007 2025-01-29T12:58:59Z 2025-01-29T12:58:59Z Journal Article https://hdl.handle.net/10568/171920 en Open Access Public Library of Science Diretto, Gianfranco; Al-Babili, Salim; Tavazza, Raffaela; Papacchioli, Velia; Beyer, Peter; Giuliano, Giovanni. 2007. Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway. PLoS ONE Apr 4(2): e350. https://doi.org/10.1371/journal.pone.0000350 |
| spellingShingle | potatoes carotenoids retinol plant breeding Diretto, Gianfranco Al-Babili, Salim Tavazza, Raffaela Papacchioli, Velia Beyer, Peter Giuliano, Giovanni Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway |
| title | Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway |
| title_full | Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway |
| title_fullStr | Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway |
| title_full_unstemmed | Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway |
| title_short | Metabolic engineering of potato carotenoid content through tuber-specific overexpression of a bacterial mini-pathway |
| title_sort | metabolic engineering of potato carotenoid content through tuber specific overexpression of a bacterial mini pathway |
| topic | potatoes carotenoids retinol plant breeding |
| url | https://hdl.handle.net/10568/171920 |
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