Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme
Vitamin A deficiency is a global health problem and can be effectively alleviated through crop biofortification. Quantification of carotenoids using high-performance liquid chromatography is expensive and time-consuming, thereby posing a challenge in the selection of genotypes with high provitamin A...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Informa UK Limited
2015
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| Acceso en línea: | https://hdl.handle.net/10568/170939 |
| _version_ | 1855541607428784128 |
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| author | Muthusamy, Vignesh Hossain, Firoz Thirunavukkarasu, Nepolean Saha, Supradip Shanker, Hari |
| author_browse | Hossain, Firoz Muthusamy, Vignesh Saha, Supradip Shanker, Hari Thirunavukkarasu, Nepolean |
| author_facet | Muthusamy, Vignesh Hossain, Firoz Thirunavukkarasu, Nepolean Saha, Supradip Shanker, Hari |
| author_sort | Muthusamy, Vignesh |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Vitamin A deficiency is a global health problem and can be effectively alleviated through crop biofortification. Quantification of carotenoids using high-performance liquid chromatography is expensive and time-consuming, thereby posing a challenge in the selection of genotypes with high provitamin A. Favourable alleles possessing rare genetic variation in lycopene-ε-cyclase (lcyE) and β-carotene hydroxylase (crtRB1) genes are associated with higher accumulation of provitamin A, especially β-carotene; and selection of these alleles holds immense promise in reducing large-scale phenotypic assays. Screening of a diverse set of 385 maize inbred lines of indigenous and exotic origin detected the presence of two alleles (amplicon size: 250 and 650 bp) of lcyE and three alleles (amplicon size: 296, 543 and 875 bp) of crtRB1 in the inbred panel. Favourable alleles of both the genes were rare among the traditional maize germplasm; 3.38% of the inbreds possessed the favourable allele (650 bp) of lcyE, while 3.90% inbreds had the favourable allele (543 bp) of crtRB1. Five inbreds (1.3%) with favourable alleles of both the genes were found. Inbreds with favourable alleles of crtRB1 and lcyE serve as rich genetic resources for effective utilization in the maize biofortification programme. |
| format | Journal Article |
| id | CGSpace170939 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace1709392025-09-25T13:01:45Z Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme Muthusamy, Vignesh Hossain, Firoz Thirunavukkarasu, Nepolean Saha, Supradip Shanker, Hari retinol biofortification Vitamin A deficiency is a global health problem and can be effectively alleviated through crop biofortification. Quantification of carotenoids using high-performance liquid chromatography is expensive and time-consuming, thereby posing a challenge in the selection of genotypes with high provitamin A. Favourable alleles possessing rare genetic variation in lycopene-ε-cyclase (lcyE) and β-carotene hydroxylase (crtRB1) genes are associated with higher accumulation of provitamin A, especially β-carotene; and selection of these alleles holds immense promise in reducing large-scale phenotypic assays. Screening of a diverse set of 385 maize inbred lines of indigenous and exotic origin detected the presence of two alleles (amplicon size: 250 and 650 bp) of lcyE and three alleles (amplicon size: 296, 543 and 875 bp) of crtRB1 in the inbred panel. Favourable alleles of both the genes were rare among the traditional maize germplasm; 3.38% of the inbreds possessed the favourable allele (650 bp) of lcyE, while 3.90% inbreds had the favourable allele (543 bp) of crtRB1. Five inbreds (1.3%) with favourable alleles of both the genes were found. Inbreds with favourable alleles of crtRB1 and lcyE serve as rich genetic resources for effective utilization in the maize biofortification programme. 2015-12-31 2025-01-29T12:57:30Z 2025-01-29T12:57:30Z Journal Article https://hdl.handle.net/10568/170939 en Open Access Informa UK Limited Muthusamy, Vignesh; Hossain, Firoz; Thirunavukkarasu, Nepolean; Saha, Supradip; Shanker, Hari. 2015. Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme. Cogent Food & Agriculture 1(1). https://doi.org/10.1080/23311932.2015.1033141 |
| spellingShingle | retinol biofortification Muthusamy, Vignesh Hossain, Firoz Thirunavukkarasu, Nepolean Saha, Supradip Shanker, Hari Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme |
| title | Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme |
| title_full | Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme |
| title_fullStr | Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme |
| title_full_unstemmed | Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme |
| title_short | Allelic variations for lycopene-ε-cyclase and β-carotene hydroxylase genes in maize inbreds and their utilization in β-carotene enrichment programme |
| title_sort | allelic variations for lycopene ε cyclase and β carotene hydroxylase genes in maize inbreds and their utilization in β carotene enrichment programme |
| topic | retinol biofortification |
| url | https://hdl.handle.net/10568/170939 |
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