Gelatinization temperature of rice explained by polymorphisms in starch synthase

The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice st...

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Main Authors: Waters, Daniel L. E., Henry, Robert J., Reinke, Russell F., Fitzgerald, Melissa A.
Format: Journal Article
Language:Inglés
Published: Wiley 2006
Subjects:
Online Access:https://hdl.handle.net/10568/166694
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author Waters, Daniel L. E.
Henry, Robert J.
Reinke, Russell F.
Fitzgerald, Melissa A.
author_browse Fitzgerald, Melissa A.
Henry, Robert J.
Reinke, Russell F.
Waters, Daniel L. E.
author_facet Waters, Daniel L. E.
Henry, Robert J.
Reinke, Russell F.
Fitzgerald, Melissa A.
author_sort Waters, Daniel L. E.
collection Repository of Agricultural Research Outputs (CGSpace)
description The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence analysis of the eight exons of SSIIa identified significant polymorphism in only exon 8. These single nucleotide polymorphisms (SNPs) were determined in 70 diverse genotypes of rice. Two SNPs could classify all 70 genotypes into either high GT or low GT types which differed in GT by 8 °C. ‘A’ rather than ‘G’ at base 2412 determined whether a methionine or valine was present at the corresponding amino acid residue in SSIIa, whilst two adjacent SNPs at bases 2543 and 2544 coded for either leucine (GC) or phenylalanine (TT). Rice varieties with high GT starch had a combination of valine and leucine at these residues. In contrast, rice varieties with low GT starch had a combination of either methionine and leucine or valine and phenylalanine at these same residues. At least two distinct polymorphisms have apparently been selected for their desirable cooking qualities in the domestication of rice.
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spelling CGSpace1666942024-12-19T14:13:31Z Gelatinization temperature of rice explained by polymorphisms in starch synthase Waters, Daniel L. E. Henry, Robert J. Reinke, Russell F. Fitzgerald, Melissa A. cooking quality exons genotypes leucine methionine nucleotide sequences phenylalanine starch valine The cooking quality of rice is associated with the starch gelatinization temperature (GT). Rice genotypes with low GT have probably been selected for their cooking quality by humans during domestication. We now report polymorphisms in starch synthase IIa (SSIIa) that explain the variation in rice starch GT. Sequence analysis of the eight exons of SSIIa identified significant polymorphism in only exon 8. These single nucleotide polymorphisms (SNPs) were determined in 70 diverse genotypes of rice. Two SNPs could classify all 70 genotypes into either high GT or low GT types which differed in GT by 8 °C. ‘A’ rather than ‘G’ at base 2412 determined whether a methionine or valine was present at the corresponding amino acid residue in SSIIa, whilst two adjacent SNPs at bases 2543 and 2544 coded for either leucine (GC) or phenylalanine (TT). Rice varieties with high GT starch had a combination of valine and leucine at these residues. In contrast, rice varieties with low GT starch had a combination of either methionine and leucine or valine and phenylalanine at these same residues. At least two distinct polymorphisms have apparently been selected for their desirable cooking qualities in the domestication of rice. 2006-01 2024-12-19T12:56:34Z 2024-12-19T12:56:34Z Journal Article https://hdl.handle.net/10568/166694 en Wiley Waters, Daniel L. E.; Henry, Robert J.; Reinke, Russell F. and Fitzgerald, Melissa A. 2006. Gelatinization temperature of rice explained by polymorphisms in starch synthase. Plant Biotechnology Journal, Volume 4 no. 1 p. 115-122
spellingShingle cooking quality
exons
genotypes
leucine
methionine
nucleotide sequences
phenylalanine
starch
valine
Waters, Daniel L. E.
Henry, Robert J.
Reinke, Russell F.
Fitzgerald, Melissa A.
Gelatinization temperature of rice explained by polymorphisms in starch synthase
title Gelatinization temperature of rice explained by polymorphisms in starch synthase
title_full Gelatinization temperature of rice explained by polymorphisms in starch synthase
title_fullStr Gelatinization temperature of rice explained by polymorphisms in starch synthase
title_full_unstemmed Gelatinization temperature of rice explained by polymorphisms in starch synthase
title_short Gelatinization temperature of rice explained by polymorphisms in starch synthase
title_sort gelatinization temperature of rice explained by polymorphisms in starch synthase
topic cooking quality
exons
genotypes
leucine
methionine
nucleotide sequences
phenylalanine
starch
valine
url https://hdl.handle.net/10568/166694
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AT fitzgeraldmelissaa gelatinizationtemperatureofriceexplainedbypolymorphismsinstarchsynthase