Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.)
Six simple methods for extracting DNA from rice seedlings were evaluated for marker‐assisted selection (MAS). The assessment of each method was based on PCR amplification of SSR markers, DNA yield and purity, time and cost. Based on these criteria, two methods were selected as being superior to othe...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2007
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/166486 |
| _version_ | 1855514164870512640 |
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| author | Collard, B. C. Y. Das, A. Virk, P.S. Mackill, D.J. |
| author_browse | Collard, B. C. Y. Das, A. Mackill, D.J. Virk, P.S. |
| author_facet | Collard, B. C. Y. Das, A. Virk, P.S. Mackill, D.J. |
| author_sort | Collard, B. C. Y. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Six simple methods for extracting DNA from rice seedlings were evaluated for marker‐assisted selection (MAS). The assessment of each method was based on PCR amplification of SSR markers, DNA yield and purity, time and cost. Based on these criteria, two methods were selected as being superior to other methods. The best two methods included the standard method developed at the International Rice Research Institute (IRRI), which utilizes a sodium dodecyl sulfate extraction buffer followed by chloroform/isoamyl alcohol extraction and a previously published method using sodium hydroxide and Tris. These two methods produced nearly identical PCR amplification results. The sodium hydroxide method is considerably simpler, quicker and cheaper than the standard IRRI method, and may be particularly useful for many applications of MAS or high‐resolution mapping. This method was also adapted into an effective high throughput method utilizing 96‐well plates emphasizing its versatility. |
| format | Journal Article |
| id | CGSpace166486 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace1664862025-05-14T10:39:27Z Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) Collard, B. C. Y. Das, A. Virk, P.S. Mackill, D.J. dna genes genetic markers polymerase chain reaction selection simple sequence repeats Six simple methods for extracting DNA from rice seedlings were evaluated for marker‐assisted selection (MAS). The assessment of each method was based on PCR amplification of SSR markers, DNA yield and purity, time and cost. Based on these criteria, two methods were selected as being superior to other methods. The best two methods included the standard method developed at the International Rice Research Institute (IRRI), which utilizes a sodium dodecyl sulfate extraction buffer followed by chloroform/isoamyl alcohol extraction and a previously published method using sodium hydroxide and Tris. These two methods produced nearly identical PCR amplification results. The sodium hydroxide method is considerably simpler, quicker and cheaper than the standard IRRI method, and may be particularly useful for many applications of MAS or high‐resolution mapping. This method was also adapted into an effective high throughput method utilizing 96‐well plates emphasizing its versatility. 2007-02 2024-12-19T12:56:20Z 2024-12-19T12:56:20Z Journal Article https://hdl.handle.net/10568/166486 en Wiley Collard, B. C. Y.; Das, A.; Virk, P. S. and Mackill, D. J. 2007. Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.). Plant Breeding, Volume 126 no. 1 p. 47-50 |
| spellingShingle | dna genes genetic markers polymerase chain reaction selection simple sequence repeats Collard, B. C. Y. Das, A. Virk, P.S. Mackill, D.J. Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) |
| title | Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) |
| title_full | Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) |
| title_fullStr | Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) |
| title_full_unstemmed | Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) |
| title_short | Evaluation of quick and dirty DNA extraction methods for marker-assisted selection in rice (Oryza sativa L.) |
| title_sort | evaluation of quick and dirty dna extraction methods for marker assisted selection in rice oryza sativa l |
| topic | dna genes genetic markers polymerase chain reaction selection simple sequence repeats |
| url | https://hdl.handle.net/10568/166486 |
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