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Genotyping SSR length variants by isothermal DNA amplification

Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be co...

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Main Authors: Lee, David, Ialicicco, Manuela, Akkinepalli, Harika, Morreale, Giacomo, Liu, Yan, Leung, Hei, Scippa, Gabriella S., Greenland, Andy, Mackay, Ian
Format: Journal Article
Language:Inglés
Published: Canadian Science Publishing 2012
Subjects:
dna
alleles
dna amplification
dna sequencing
genetic markers
genotypes
methods
microsatellites
nucleotide sequences
polymerase chain reaction
simple sequence repeats
varieties
Online Access:https://hdl.handle.net/10568/165746
Summary:Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.

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