Genotyping SSR length variants by isothermal DNA amplification
Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be co...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Canadian Science Publishing
2012
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/165746 |
| _version_ | 1855539261400416256 |
|---|---|
| author | Lee, David Ialicicco, Manuela Akkinepalli, Harika Morreale, Giacomo Liu, Yan Leung, Hei Scippa, Gabriella S. Greenland, Andy Mackay, Ian |
| author_browse | Akkinepalli, Harika Greenland, Andy Ialicicco, Manuela Lee, David Leung, Hei Liu, Yan Mackay, Ian Morreale, Giacomo Scippa, Gabriella S. |
| author_facet | Lee, David Ialicicco, Manuela Akkinepalli, Harika Morreale, Giacomo Liu, Yan Leung, Hei Scippa, Gabriella S. Greenland, Andy Mackay, Ian |
| author_sort | Lee, David |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target. |
| format | Journal Article |
| id | CGSpace165746 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Canadian Science Publishing |
| publisherStr | Canadian Science Publishing |
| record_format | dspace |
| spelling | CGSpace1657462025-12-08T09:54:28Z Genotyping SSR length variants by isothermal DNA amplification Lee, David Ialicicco, Manuela Akkinepalli, Harika Morreale, Giacomo Liu, Yan Leung, Hei Scippa, Gabriella S. Greenland, Andy Mackay, Ian dna alleles dna amplification dna sequencing genetic markers genotypes methods microsatellites nucleotide sequences polymerase chain reaction simple sequence repeats varieties Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target. 2012-09 2024-12-19T12:55:25Z 2024-12-19T12:55:25Z Journal Article https://hdl.handle.net/10568/165746 en Canadian Science Publishing Lee, David; Ialicicco, Manuela; Akkinepalli, Harika; Morreale, Giacomo; Liu, Yan; Leung, Hei; Scippa, Gabriella S.; Greenland, Andy and Mackay, Ian. 2012. Genotyping SSR length variants by isothermal DNA amplification. Genome, Volume 55 no. 09 p. 691-695 |
| spellingShingle | dna alleles dna amplification dna sequencing genetic markers genotypes methods microsatellites nucleotide sequences polymerase chain reaction simple sequence repeats varieties Lee, David Ialicicco, Manuela Akkinepalli, Harika Morreale, Giacomo Liu, Yan Leung, Hei Scippa, Gabriella S. Greenland, Andy Mackay, Ian Genotyping SSR length variants by isothermal DNA amplification |
| title | Genotyping SSR length variants by isothermal DNA amplification |
| title_full | Genotyping SSR length variants by isothermal DNA amplification |
| title_fullStr | Genotyping SSR length variants by isothermal DNA amplification |
| title_full_unstemmed | Genotyping SSR length variants by isothermal DNA amplification |
| title_short | Genotyping SSR length variants by isothermal DNA amplification |
| title_sort | genotyping ssr length variants by isothermal dna amplification |
| topic | dna alleles dna amplification dna sequencing genetic markers genotypes methods microsatellites nucleotide sequences polymerase chain reaction simple sequence repeats varieties |
| url | https://hdl.handle.net/10568/165746 |
| work_keys_str_mv | AT leedavid genotypingssrlengthvariantsbyisothermaldnaamplification AT ialiciccomanuela genotypingssrlengthvariantsbyisothermaldnaamplification AT akkinepalliharika genotypingssrlengthvariantsbyisothermaldnaamplification AT morrealegiacomo genotypingssrlengthvariantsbyisothermaldnaamplification AT liuyan genotypingssrlengthvariantsbyisothermaldnaamplification AT leunghei genotypingssrlengthvariantsbyisothermaldnaamplification AT scippagabriellas genotypingssrlengthvariantsbyisothermaldnaamplification AT greenlandandy genotypingssrlengthvariantsbyisothermaldnaamplification AT mackayian genotypingssrlengthvariantsbyisothermaldnaamplification |