Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination
Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study evaluated t...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2017
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| Acceso en línea: | https://hdl.handle.net/10568/165021 |
| _version_ | 1855539344264134656 |
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| author | Guzman, Maria Krishna de Parween, Sabiha Butardo, Vito M. Alhambra, Crisline Mae Anacleto, Roslen Seiler, Christiane Bird, Anthony R. Chow, Chung-Ping Sreenivasulu, Nese |
| author_browse | Alhambra, Crisline Mae Anacleto, Roslen Bird, Anthony R. Butardo, Vito M. Chow, Chung-Ping Guzman, Maria Krishna de Parween, Sabiha Seiler, Christiane Sreenivasulu, Nese |
| author_facet | Guzman, Maria Krishna de Parween, Sabiha Butardo, Vito M. Alhambra, Crisline Mae Anacleto, Roslen Seiler, Christiane Bird, Anthony R. Chow, Chung-Ping Sreenivasulu, Nese |
| author_sort | Guzman, Maria Krishna de |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study evaluated the feasibility of using in vitro cooked grain amylolysis, starch mobilization patterns during seed germination, and variation in starch structure and composition in the mature seed to differentiate patterns of starch digestibility. Mobilization patterns of total starch, resistant starch, amylose and amylopectin chains, and free sugars during seed germination revealed that the process is analogous to digestion in the human gastrointestinal tract. The combination of these biochemical markers can be used as an alternative measure to predict GI. Additionally, transcriptome analysis of stored mRNA transcripts in high and low GI lines detected differences in starch metabolism and confirmed the importance of seed storage pathways in influencing digestibility. Pathway analyses supported by metabolomics data revealed that resistant starch, cell wall non-starch polysaccharides and flavonoids potentially contribute to slower digestibility. These new insights can guide precision breeding programs to produce low GI rice with acceptable cooking quality to help mitigate the burden of diet-associated lifestyle diseases. |
| format | Journal Article |
| id | CGSpace165021 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace1650212024-12-19T14:13:34Z Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination Guzman, Maria Krishna de Parween, Sabiha Butardo, Vito M. Alhambra, Crisline Mae Anacleto, Roslen Seiler, Christiane Bird, Anthony R. Chow, Chung-Ping Sreenivasulu, Nese Rice lines with slower starch digestibility provide opportunities in mitigating the global rise in type II diabetes and related non-communicable diseases. However, screening for low glycemic index (GI) in rice breeding programs is not possible due to time and cost constraints. This study evaluated the feasibility of using in vitro cooked grain amylolysis, starch mobilization patterns during seed germination, and variation in starch structure and composition in the mature seed to differentiate patterns of starch digestibility. Mobilization patterns of total starch, resistant starch, amylose and amylopectin chains, and free sugars during seed germination revealed that the process is analogous to digestion in the human gastrointestinal tract. The combination of these biochemical markers can be used as an alternative measure to predict GI. Additionally, transcriptome analysis of stored mRNA transcripts in high and low GI lines detected differences in starch metabolism and confirmed the importance of seed storage pathways in influencing digestibility. Pathway analyses supported by metabolomics data revealed that resistant starch, cell wall non-starch polysaccharides and flavonoids potentially contribute to slower digestibility. These new insights can guide precision breeding programs to produce low GI rice with acceptable cooking quality to help mitigate the burden of diet-associated lifestyle diseases. 2017-07-19 2024-12-19T12:54:37Z 2024-12-19T12:54:37Z Journal Article https://hdl.handle.net/10568/165021 en Open Access Springer Guzman, Maria Krishna de; Parween, Sabiha; Butardo, Vito M.; Alhambra, Crisline Mae; Anacleto, Roslen; Seiler, Christiane; Bird, Anthony R.; Chow, Chung-Ping and Sreenivasulu, Nese. 2017. Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination. Sci Rep, Volume 7, no. 1 |
| spellingShingle | Guzman, Maria Krishna de Parween, Sabiha Butardo, Vito M. Alhambra, Crisline Mae Anacleto, Roslen Seiler, Christiane Bird, Anthony R. Chow, Chung-Ping Sreenivasulu, Nese Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| title | Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| title_full | Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| title_fullStr | Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| title_full_unstemmed | Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| title_short | Investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| title_sort | investigating glycemic potential of rice by unraveling compositional variations in mature grain and starch mobilization patterns during seed germination |
| url | https://hdl.handle.net/10568/165021 |
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