Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content

Rice eating and cooking quality and protein content (PC) are important properties affecting consumers’ preferences, nutrition and health. Linkage QTL mapping and association studies are usually applied to genetically dissect related traits, which could be further facilitated by high density SNP mark...

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Autores principales: Wang, Xiaoqian, Pang, Yunlong, Zhang, Jian, Wu, Zhichao, Chen, Kai, Ali, Jauhar, Ye, Guoyou, Xu, Jianlong, Li, Zhikang
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2017
Acceso en línea:https://hdl.handle.net/10568/164935
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author Wang, Xiaoqian
Pang, Yunlong
Zhang, Jian
Wu, Zhichao
Chen, Kai
Ali, Jauhar
Ye, Guoyou
Xu, Jianlong
Li, Zhikang
author_browse Ali, Jauhar
Chen, Kai
Li, Zhikang
Pang, Yunlong
Wang, Xiaoqian
Wu, Zhichao
Xu, Jianlong
Ye, Guoyou
Zhang, Jian
author_facet Wang, Xiaoqian
Pang, Yunlong
Zhang, Jian
Wu, Zhichao
Chen, Kai
Ali, Jauhar
Ye, Guoyou
Xu, Jianlong
Li, Zhikang
author_sort Wang, Xiaoqian
collection Repository of Agricultural Research Outputs (CGSpace)
description Rice eating and cooking quality and protein content (PC) are important properties affecting consumers’ preferences, nutrition and health. Linkage QTL mapping and association studies are usually applied to genetically dissect related traits, which could be further facilitated by high density SNP markers and gene annotation based on reference genome to rapid identify candidate genes associated with interested traits. Here, we carried out an association study for apparent amylose content (AC), gel consistency (GC), gelatinization temperature (GT) and PC evaluated in two environments using a diverse panel of 258 accessions from 3 K Rice Genome Project. Wide phenotypic variations were observed in this panel. Genome-wide association study using 22,488 high quality SNPs identified 19 QTL affecting the four traits. Combining gene-based association study and haplotype analyses plus functional annotation allowed us to shortlist nine candidate genes for four important QTL regions affecting AC, GC and GT, including two cloned genes (Wx and ALK), and seven novels. The research suggested that GWAS and gene-based association analysis followed by haplotype analysis is an effective way to detect candidate genes. The identified genes and QTL provided valuable sources for future functional characterization and genetic improvement of rice eating and cooking quality and PC.
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spelling CGSpace1649352024-12-19T14:13:28Z Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content Wang, Xiaoqian Pang, Yunlong Zhang, Jian Wu, Zhichao Chen, Kai Ali, Jauhar Ye, Guoyou Xu, Jianlong Li, Zhikang Rice eating and cooking quality and protein content (PC) are important properties affecting consumers’ preferences, nutrition and health. Linkage QTL mapping and association studies are usually applied to genetically dissect related traits, which could be further facilitated by high density SNP markers and gene annotation based on reference genome to rapid identify candidate genes associated with interested traits. Here, we carried out an association study for apparent amylose content (AC), gel consistency (GC), gelatinization temperature (GT) and PC evaluated in two environments using a diverse panel of 258 accessions from 3 K Rice Genome Project. Wide phenotypic variations were observed in this panel. Genome-wide association study using 22,488 high quality SNPs identified 19 QTL affecting the four traits. Combining gene-based association study and haplotype analyses plus functional annotation allowed us to shortlist nine candidate genes for four important QTL regions affecting AC, GC and GT, including two cloned genes (Wx and ALK), and seven novels. The research suggested that GWAS and gene-based association analysis followed by haplotype analysis is an effective way to detect candidate genes. The identified genes and QTL provided valuable sources for future functional characterization and genetic improvement of rice eating and cooking quality and PC. 2017-12-08 2024-12-19T12:54:28Z 2024-12-19T12:54:28Z Journal Article https://hdl.handle.net/10568/164935 en Open Access Springer Wang, Xiaoqian; Pang, Yunlong; Zhang, Jian; Wu, Zhichao; Chen, Kai; Ali, Jauhar; Ye, Guoyou; Xu, Jianlong and Li, Zhikang. 2017. Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content. Sci Rep, Volume 7, no. 1
spellingShingle Wang, Xiaoqian
Pang, Yunlong
Zhang, Jian
Wu, Zhichao
Chen, Kai
Ali, Jauhar
Ye, Guoyou
Xu, Jianlong
Li, Zhikang
Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content
title Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content
title_full Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content
title_fullStr Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content
title_full_unstemmed Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content
title_short Genome-wide and gene-based association mapping for rice eating and cooking characteristics and protein content
title_sort genome wide and gene based association mapping for rice eating and cooking characteristics and protein content
url https://hdl.handle.net/10568/164935
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