Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I)
Amylose content (AC) is the key determinant of eating and cooking quality (ECQ) of rice. The major Wxa allele of granule-bound starch synthase I (GBSS I) in indica rice produces higher AC, making rice hard and dry after cooking. Recent work has improved ECQ of japonica rice via clustered regularly i...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/164204 |
| _version_ | 1855515835173437440 |
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| author | Monsur, Mahmuda Binte Ni, Cao Xiangjin, Wei Lihong, Xie Guiai, Jiao Tang, Shaoqing Sreenivasulu, Nese Gaoneng, Shao Peisong, Hu |
| author_browse | Gaoneng, Shao Guiai, Jiao Lihong, Xie Monsur, Mahmuda Binte Ni, Cao Peisong, Hu Sreenivasulu, Nese Tang, Shaoqing Xiangjin, Wei |
| author_facet | Monsur, Mahmuda Binte Ni, Cao Xiangjin, Wei Lihong, Xie Guiai, Jiao Tang, Shaoqing Sreenivasulu, Nese Gaoneng, Shao Peisong, Hu |
| author_sort | Monsur, Mahmuda Binte |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Amylose content (AC) is the key determinant of eating and cooking quality (ECQ) of rice. The major Wxa allele of granule-bound starch synthase I (GBSS I) in indica rice produces higher AC, making rice hard and dry after cooking. Recent work has improved ECQ of japonica rice via clustered regularly interspaced short palindromic repeats/CRISPR- associated protein 9 (CRISPR/Cas9) or cytosine base editing (CBE) techniques. However, base editing has not yet been applied to modify the Wxa allele of indica rice. We utilized a novel precise adenine base editing (ABE) tool to generate three mutants of indica cultivar Zhongjiazao 17 (YK17) with reduced AC while other ECQ parameters, such as gel consistency (GC) and alkali spreading value, were maintained. Our study demonstrated improvement of ECQ of indica rice and will help rice breeders satisfy consumers. |
| format | Journal Article |
| id | CGSpace164204 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace1642042025-05-14T10:23:52Z Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) Monsur, Mahmuda Binte Ni, Cao Xiangjin, Wei Lihong, Xie Guiai, Jiao Tang, Shaoqing Sreenivasulu, Nese Gaoneng, Shao Peisong, Hu plant science agronomy and crop science biotechnology Amylose content (AC) is the key determinant of eating and cooking quality (ECQ) of rice. The major Wxa allele of granule-bound starch synthase I (GBSS I) in indica rice produces higher AC, making rice hard and dry after cooking. Recent work has improved ECQ of japonica rice via clustered regularly interspaced short palindromic repeats/CRISPR- associated protein 9 (CRISPR/Cas9) or cytosine base editing (CBE) techniques. However, base editing has not yet been applied to modify the Wxa allele of indica rice. We utilized a novel precise adenine base editing (ABE) tool to generate three mutants of indica cultivar Zhongjiazao 17 (YK17) with reduced AC while other ECQ parameters, such as gel consistency (GC) and alkali spreading value, were maintained. Our study demonstrated improvement of ECQ of indica rice and will help rice breeders satisfy consumers. 2021-09 2024-12-19T12:53:36Z 2024-12-19T12:53:36Z Journal Article https://hdl.handle.net/10568/164204 en Open Access Elsevier Monsur, Mahmuda Binte; Ni, Cao; Xiangjin, Wei; Lihong, Xie; Guiai, Jiao; Shaoqing, Tang; Sreenivasulu, Nese; Gaoneng, Shao and Peisong, Hu. 2021. Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I). Rice Science, Volume 28 no. 5 p. 427-430 |
| spellingShingle | plant science agronomy and crop science biotechnology Monsur, Mahmuda Binte Ni, Cao Xiangjin, Wei Lihong, Xie Guiai, Jiao Tang, Shaoqing Sreenivasulu, Nese Gaoneng, Shao Peisong, Hu Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) |
| title | Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) |
| title_full | Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) |
| title_fullStr | Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) |
| title_full_unstemmed | Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) |
| title_short | Improved eating and cooking quality of indica rice cultivar YK17 via adenine base editing of Wx(a) allele of Granule-Bound Starch Synthase I (GBSS I) |
| title_sort | improved eating and cooking quality of indica rice cultivar yk17 via adenine base editing of wx a allele of granule bound starch synthase i gbss i |
| topic | plant science agronomy and crop science biotechnology |
| url | https://hdl.handle.net/10568/164204 |
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