Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures
Progressive loss of plant diversity requires the protection of wild and agri-/horticultural species. For species whose seeds are extremely short-lived, or rarely or never produce seeds, or whose genetic makeup must be preserved, cryopreservation offers the only possibility for long-term conservation...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Annual Reviews
2024
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/149292 |
| _version_ | 1855520502728097792 |
|---|---|
| author | Nagel, Manuela Pence, Valerie Ballesteros, Daniel Lambardi, Maurizio Popova, Elena Panis, Bart |
| author_browse | Ballesteros, Daniel Lambardi, Maurizio Nagel, Manuela Panis, Bart Pence, Valerie Popova, Elena |
| author_facet | Nagel, Manuela Pence, Valerie Ballesteros, Daniel Lambardi, Maurizio Popova, Elena Panis, Bart |
| author_sort | Nagel, Manuela |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Progressive loss of plant diversity requires the protection of wild and agri-/horticultural species. For species whose seeds are extremely short-lived, or rarely or never produce seeds, or whose genetic makeup must be preserved, cryopreservation offers the only possibility for long-term conservation. At temperatures below freezing, most vegetative plant tissues suffer severe damage from ice crystal formation and require protection. In this review, we describe how increasing the concentration of cellular solutes by air drying or adding cryoprotectants, together with rapid cooling, results in a vitrified, highly viscous state in which cells can remain viable and be stored. On this basis, a range of dormant bud–freezing, slow-cooling, and (droplet-)vitrification protocols have been developed, but few are used to cryobank important agricultural/horticultural/timber and threatened species. To improve cryopreservation efficiency, the effects of cryoprotectants and molecular processes need to be understood and the costs for cryobanking reduced. However, overall, the long-term costs of cryopreservation are low, while the benefits are huge. Expected final online publication date for the Annual Review of Plant Biology, Volume 75 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates. |
| format | Journal Article |
| id | CGSpace149292 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Annual Reviews |
| publisherStr | Annual Reviews |
| record_format | dspace |
| spelling | CGSpace1492922025-12-08T09:54:28Z Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures Nagel, Manuela Pence, Valerie Ballesteros, Daniel Lambardi, Maurizio Popova, Elena Panis, Bart evaluation gene banks genetic resources conservation cryopreservation vitrification vitroplants liquid nitrogen Progressive loss of plant diversity requires the protection of wild and agri-/horticultural species. For species whose seeds are extremely short-lived, or rarely or never produce seeds, or whose genetic makeup must be preserved, cryopreservation offers the only possibility for long-term conservation. At temperatures below freezing, most vegetative plant tissues suffer severe damage from ice crystal formation and require protection. In this review, we describe how increasing the concentration of cellular solutes by air drying or adding cryoprotectants, together with rapid cooling, results in a vitrified, highly viscous state in which cells can remain viable and be stored. On this basis, a range of dormant bud–freezing, slow-cooling, and (droplet-)vitrification protocols have been developed, but few are used to cryobank important agricultural/horticultural/timber and threatened species. To improve cryopreservation efficiency, the effects of cryoprotectants and molecular processes need to be understood and the costs for cryobanking reduced. However, overall, the long-term costs of cryopreservation are low, while the benefits are huge. Expected final online publication date for the Annual Review of Plant Biology, Volume 75 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates. 2024-07-22 2024-07-29T10:13:25Z 2024-07-29T10:13:25Z Journal Article https://hdl.handle.net/10568/149292 en Open Access application/pdf Annual Reviews Nagel, M.; Pence, V.; Ballesteros, D.; Lambardi, M.; Popova, E.; Panis, B. (2024) Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures. Annual Review of Plant Biology 75(1): p. 797-824 . ISSN: 1543-5008 |
| spellingShingle | evaluation gene banks genetic resources conservation cryopreservation vitrification vitroplants liquid nitrogen Nagel, Manuela Pence, Valerie Ballesteros, Daniel Lambardi, Maurizio Popova, Elena Panis, Bart Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures |
| title | Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures |
| title_full | Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures |
| title_fullStr | Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures |
| title_full_unstemmed | Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures |
| title_short | Plant cryopreservation: Principles, applications, and challenges of banking plant diversity at ultralow temperatures |
| title_sort | plant cryopreservation principles applications and challenges of banking plant diversity at ultralow temperatures |
| topic | evaluation gene banks genetic resources conservation cryopreservation vitrification vitroplants liquid nitrogen |
| url | https://hdl.handle.net/10568/149292 |
| work_keys_str_mv | AT nagelmanuela plantcryopreservationprinciplesapplicationsandchallengesofbankingplantdiversityatultralowtemperatures AT pencevalerie plantcryopreservationprinciplesapplicationsandchallengesofbankingplantdiversityatultralowtemperatures AT ballesterosdaniel plantcryopreservationprinciplesapplicationsandchallengesofbankingplantdiversityatultralowtemperatures AT lambardimaurizio plantcryopreservationprinciplesapplicationsandchallengesofbankingplantdiversityatultralowtemperatures AT popovaelena plantcryopreservationprinciplesapplicationsandchallengesofbankingplantdiversityatultralowtemperatures AT panisbart plantcryopreservationprinciplesapplicationsandchallengesofbankingplantdiversityatultralowtemperatures |