Genomic surveillance of Rift Valley fever virus

Pathogen genomic data are critical in the development of vaccines and diagnostics. Currently, there are only a handful of Rift Valley fever virus (RVFV) genomic sequence data. For a long time, generation of genomic data for RVFV has relied on culture of the virus. This procedure often requires hig...

Descripción completa

Detalles Bibliográficos
Autores principales: Juma, John, Limbaso, S.K.
Formato: Póster
Lenguaje:Inglés
Publicado: ILRI 2024
Materias:
Acceso en línea:https://hdl.handle.net/10568/140467
Descripción
Sumario:Pathogen genomic data are critical in the development of vaccines and diagnostics. Currently, there are only a handful of Rift Valley fever virus (RVFV) genomic sequence data. For a long time, generation of genomic data for RVFV has relied on culture of the virus. This procedure often requires highly skilled personnel to handle the pathogenic virus, and is labour-intensive and time consuming (minimum 14 days). We designed a simple and cost-effective method that enriches the virus particles directly from clinical samples. This enrichment procedure targets the virus, which can then be sequenced in a near real-time manner devoid of cell culture enrichment.