Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives
Grasspea (Lathyrus sativus L.) is recognized as a highly drought-tolerant legume. However, excessive consumption of its seeds and green tissues causes neurolathyrism, a condition characterized by an irreversible paralysis of the legs induced by a neurotoxin amino acid called β-N-oxalyl-L-α, β- diami...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/132605 |
| _version_ | 1855518494306598912 |
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| author | Aloui, Khawla Choukri, Hasnae El Haddad, Noureddine Gupta, Priyanka Bouhmadi, Keltoum Emmrich, Peter M. F. Singh, Akanksha Edwards, Anne Maalouf, Fouad Bouhlal, Outmane Staples, Jasmine Agrawal, Shiv Kumar |
| author_browse | Agrawal, Shiv Kumar Aloui, Khawla Bouhlal, Outmane Bouhmadi, Keltoum Choukri, Hasnae Edwards, Anne El Haddad, Noureddine Emmrich, Peter M. F. Gupta, Priyanka Maalouf, Fouad Singh, Akanksha Staples, Jasmine |
| author_facet | Aloui, Khawla Choukri, Hasnae El Haddad, Noureddine Gupta, Priyanka Bouhmadi, Keltoum Emmrich, Peter M. F. Singh, Akanksha Edwards, Anne Maalouf, Fouad Bouhlal, Outmane Staples, Jasmine Agrawal, Shiv Kumar |
| author_sort | Aloui, Khawla |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Grasspea (Lathyrus sativus L.) is recognized as a highly drought-tolerant legume. However, excessive consumption of its seeds and green tissues causes neurolathyrism, a condition characterized by an irreversible paralysis of the legs induced by a neurotoxin amino acid called β-N-oxalyl-L-α, β- diaminopropionic acid (β-ODAP). The present study investigated the effects of heat, and combined heat + drought during the reproductive phase on physiological and phenological parameters, yield-related factors, ODAP content, and seed protein of 24 genotypes representing 11 Lathyrus species under controlled conditions. Analysis of variance revealed a highly significant effect (p < 0.001) of stress treatments and genotypes for all the traits. In general, heat stress individually or in combination with drought expedited phenology, reduced relative leaf water content, stimulated proline synthesis, and influenced chlorophyll concentration; the effects were more severe under the combined heat + drought stress. ODAP content in seeds ranged from 0.06 to 0.30% under no-stress conditions. However, under heat stress, there was a significant increase of 33% in ODAP content, and under combined stress (heat + drought), the increase reached 83%. Crude protein content ranged from 15.64 to 28.67% among no stress plants and decreased significantly by 23% under heat stress and by 36% under combined stress. The findings of this study also indicated substantial reductions in growth and grain yield traits under both heat stress and combined heat + drought stress. Six accessions namely IG 66026, IG 65018, IG 65687, IG 118511, IG 64931, and IG65273 were identified as having the most favorable combination of yield, protein content, and seed ODAP levels across all conditions. ODAP content in these six accessions varied from 0.07 to 0.11% under no stress and remained at moderate levels during both heat stress (0.09–0.14%) and combined stress (0.11–0.17%). IG 66026 was identified as the most stable genotype under drought and heat stress conditions with high protein content, and low ODAP content. By identifying those promising accessions, our results have established a basis for forthcoming grasspea breeding initiatives while paving the way for future research exploration into the fundamental mechanisms driving ODAP variation in the presence of both heat and drought stress conditions. |
| format | Journal Article |
| id | CGSpace132605 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace1326052026-01-15T02:04:06Z Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives Aloui, Khawla Choukri, Hasnae El Haddad, Noureddine Gupta, Priyanka Bouhmadi, Keltoum Emmrich, Peter M. F. Singh, Akanksha Edwards, Anne Maalouf, Fouad Bouhlal, Outmane Staples, Jasmine Agrawal, Shiv Kumar crude protein drought stress heat stress grass pea grass pea (lathyrus sativus) grain yield odap content Grasspea (Lathyrus sativus L.) is recognized as a highly drought-tolerant legume. However, excessive consumption of its seeds and green tissues causes neurolathyrism, a condition characterized by an irreversible paralysis of the legs induced by a neurotoxin amino acid called β-N-oxalyl-L-α, β- diaminopropionic acid (β-ODAP). The present study investigated the effects of heat, and combined heat + drought during the reproductive phase on physiological and phenological parameters, yield-related factors, ODAP content, and seed protein of 24 genotypes representing 11 Lathyrus species under controlled conditions. Analysis of variance revealed a highly significant effect (p < 0.001) of stress treatments and genotypes for all the traits. In general, heat stress individually or in combination with drought expedited phenology, reduced relative leaf water content, stimulated proline synthesis, and influenced chlorophyll concentration; the effects were more severe under the combined heat + drought stress. ODAP content in seeds ranged from 0.06 to 0.30% under no-stress conditions. However, under heat stress, there was a significant increase of 33% in ODAP content, and under combined stress (heat + drought), the increase reached 83%. Crude protein content ranged from 15.64 to 28.67% among no stress plants and decreased significantly by 23% under heat stress and by 36% under combined stress. The findings of this study also indicated substantial reductions in growth and grain yield traits under both heat stress and combined heat + drought stress. Six accessions namely IG 66026, IG 65018, IG 65687, IG 118511, IG 64931, and IG65273 were identified as having the most favorable combination of yield, protein content, and seed ODAP levels across all conditions. ODAP content in these six accessions varied from 0.07 to 0.11% under no stress and remained at moderate levels during both heat stress (0.09–0.14%) and combined stress (0.11–0.17%). IG 66026 was identified as the most stable genotype under drought and heat stress conditions with high protein content, and low ODAP content. By identifying those promising accessions, our results have established a basis for forthcoming grasspea breeding initiatives while paving the way for future research exploration into the fundamental mechanisms driving ODAP variation in the presence of both heat and drought stress conditions. 2023-10-31T20:22:33Z 2023-10-31T20:22:33Z Journal Article https://hdl.handle.net/10568/132605 en Open Access application/pdf MDPI Khawla Aloui, Hasnae Choukri, Noureddine El Haddad, Priyanka Gupta, Keltoum Bouhmadi, Peter M. F. Emmrich, Akanksha Singh, Anne Edwards, Fouad Maalouf, Outmane Bouhlal, Jasmine Staples, Shiv Kumar Agrawal. (8/10/2023). Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives. Plants, 12 (19). |
| spellingShingle | crude protein drought stress heat stress grass pea grass pea (lathyrus sativus) grain yield odap content Aloui, Khawla Choukri, Hasnae El Haddad, Noureddine Gupta, Priyanka Bouhmadi, Keltoum Emmrich, Peter M. F. Singh, Akanksha Edwards, Anne Maalouf, Fouad Bouhlal, Outmane Staples, Jasmine Agrawal, Shiv Kumar Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives |
| title | Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives |
| title_full | Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives |
| title_fullStr | Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives |
| title_full_unstemmed | Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives |
| title_short | Impact of Heat and Drought Stress on Grasspea and Its Wild Relatives |
| title_sort | impact of heat and drought stress on grasspea and its wild relatives |
| topic | crude protein drought stress heat stress grass pea grass pea (lathyrus sativus) grain yield odap content |
| url | https://hdl.handle.net/10568/132605 |
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