Cover Image
QR Code

Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus

African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expr...

Full description

Main Authors: Feng, W., Zhou, L., Du, H., Okoth, Edward A., Mrode, Raphael A., Jin, W., Hu, Z., Liu, J.-F.
Format: Journal Article
Language:Inglés
Published: FapUNIFESP 2023
Subjects:
animal diseases
african swine fever
swine
genetics
Online Access:https://hdl.handle.net/10568/132485
_version_ 1855529393858805760
author Feng, W.
Zhou, L.
Du, H.
Okoth, Edward A.
Mrode, Raphael A.
Jin, W.
Hu, Z.
Liu, J.-F.
author_browse Du, H.
Feng, W.
Hu, Z.
Jin, W.
Liu, J.-F.
Mrode, Raphael A.
Okoth, Edward A.
Zhou, L.
author_facet Feng, W.
Zhou, L.
Du, H.
Okoth, Edward A.
Mrode, Raphael A.
Jin, W.
Hu, Z.
Liu, J.-F.
author_sort Feng, W.
collection Repository of Agricultural Research Outputs (CGSpace)
description African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expressed genes (DEGs) of six tissues from Kenya domestic pigs and Landrace × Yorkshire (L/Y) pigs infected with ASFV Kenya1033 in vivo. As results, a total of 209, 522, 34, 505, 634 and 138 DEGs (q-value < 0.05 and |Log2foldchange| values >2) were detected in the kidney, liver, mesenteric lymph node, peripheral blood mononuclear cell, submandibular lymph node and spleen, respectively. The expression profiles of DEGs shared in the multiple tissues illustrated variation in regulation function in the different tissues. Functional annotation analysis and interaction of proteins encoded by DEGs revealed that genes including IFIT1, IFITM1, MX1, OASL, ISG15, SAMHD1, IFINA1, S100A12 and S100A8 enriched in the immune and antivirus pathways were significantly changed when the hosts were infected with ASFV. The genes mentioned could play crucial roles in the process of the reaction to non-lethal ASF infection, which may will help to improve the ASF tolerance in the pig population through molecular breeding strategies.
format Journal Article
id CGSpace132485
institution CGIAR Consortium
language Inglés
publishDate 2023
publishDateRange 2023
publishDateSort 2023
publisher FapUNIFESP
publisherStr FapUNIFESP
record_format dspace
spelling CGSpace1324852025-10-26T12:53:00Z Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus Feng, W. Zhou, L. Du, H. Okoth, Edward A. Mrode, Raphael A. Jin, W. Hu, Z. Liu, J.-F. animal diseases african swine fever swine genetics African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expressed genes (DEGs) of six tissues from Kenya domestic pigs and Landrace × Yorkshire (L/Y) pigs infected with ASFV Kenya1033 in vivo. As results, a total of 209, 522, 34, 505, 634 and 138 DEGs (q-value < 0.05 and |Log2foldchange| values >2) were detected in the kidney, liver, mesenteric lymph node, peripheral blood mononuclear cell, submandibular lymph node and spleen, respectively. The expression profiles of DEGs shared in the multiple tissues illustrated variation in regulation function in the different tissues. Functional annotation analysis and interaction of proteins encoded by DEGs revealed that genes including IFIT1, IFITM1, MX1, OASL, ISG15, SAMHD1, IFINA1, S100A12 and S100A8 enriched in the immune and antivirus pathways were significantly changed when the hosts were infected with ASFV. The genes mentioned could play crucial roles in the process of the reaction to non-lethal ASF infection, which may will help to improve the ASF tolerance in the pig population through molecular breeding strategies. 2023-10-13 2023-10-26T14:42:13Z 2023-10-26T14:42:13Z Journal Article https://hdl.handle.net/10568/132485 en Open Access FapUNIFESP Feng, W., Zhou, L., Du, H., Okoth, E., Mrode, R., Jin, W., Hu, Z. and Liu, J.-F. 2023. Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus. Genetics and Molecular Biology 46(3): e20230037.
spellingShingle animal diseases
african swine fever
swine
genetics
Feng, W.
Zhou, L.
Du, H.
Okoth, Edward A.
Mrode, Raphael A.
Jin, W.
Hu, Z.
Liu, J.-F.
Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
title Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
title_full Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
title_fullStr Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
title_full_unstemmed Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
title_short Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
title_sort transcriptome analysis reveals gene expression changes of pigs infected with non lethal african swine fever virus
topic animal diseases
african swine fever
swine
genetics
url https://hdl.handle.net/10568/132485
work_keys_str_mv AT fengw transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT zhoul transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT duh transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT okothedwarda transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT mroderaphaela transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT jinw transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT huz transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus
AT liujf transcriptomeanalysisrevealsgeneexpressionchangesofpigsinfectedwithnonlethalafricanswinefevervirus

Similar Items