Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus
African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expr...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
FapUNIFESP
2023
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/132485 |
| _version_ | 1855529393858805760 |
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| author | Feng, W. Zhou, L. Du, H. Okoth, Edward A. Mrode, Raphael A. Jin, W. Hu, Z. Liu, J.-F. |
| author_browse | Du, H. Feng, W. Hu, Z. Jin, W. Liu, J.-F. Mrode, Raphael A. Okoth, Edward A. Zhou, L. |
| author_facet | Feng, W. Zhou, L. Du, H. Okoth, Edward A. Mrode, Raphael A. Jin, W. Hu, Z. Liu, J.-F. |
| author_sort | Feng, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expressed genes (DEGs) of six tissues from Kenya domestic pigs and Landrace × Yorkshire (L/Y) pigs infected with ASFV Kenya1033 in vivo. As results, a total of 209, 522, 34, 505, 634 and 138 DEGs (q-value < 0.05 and |Log2foldchange| values >2) were detected in the kidney, liver, mesenteric lymph node, peripheral blood mononuclear cell, submandibular lymph node and spleen, respectively. The expression profiles of DEGs shared in the multiple tissues illustrated variation in regulation function in the different tissues. Functional annotation analysis and interaction of proteins encoded by DEGs revealed that genes including IFIT1, IFITM1, MX1, OASL, ISG15, SAMHD1, IFINA1, S100A12 and S100A8 enriched in the immune and antivirus pathways were significantly changed when the hosts were infected with ASFV. The genes mentioned could play crucial roles in the process of the reaction to non-lethal ASF infection, which may will help to improve the ASF tolerance in the pig population through molecular breeding strategies. |
| format | Journal Article |
| id | CGSpace132485 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | FapUNIFESP |
| publisherStr | FapUNIFESP |
| record_format | dspace |
| spelling | CGSpace1324852025-10-26T12:53:00Z Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus Feng, W. Zhou, L. Du, H. Okoth, Edward A. Mrode, Raphael A. Jin, W. Hu, Z. Liu, J.-F. animal diseases african swine fever swine genetics African swine fever (ASF) is an important viral disease of swine caused by the African swine fever virus (ASFV), which threatens swine production profoundly. To better understand the gene expression changes when pig infected with ASFV, RNA sequencing was performed to characterize differentially expressed genes (DEGs) of six tissues from Kenya domestic pigs and Landrace × Yorkshire (L/Y) pigs infected with ASFV Kenya1033 in vivo. As results, a total of 209, 522, 34, 505, 634 and 138 DEGs (q-value < 0.05 and |Log2foldchange| values >2) were detected in the kidney, liver, mesenteric lymph node, peripheral blood mononuclear cell, submandibular lymph node and spleen, respectively. The expression profiles of DEGs shared in the multiple tissues illustrated variation in regulation function in the different tissues. Functional annotation analysis and interaction of proteins encoded by DEGs revealed that genes including IFIT1, IFITM1, MX1, OASL, ISG15, SAMHD1, IFINA1, S100A12 and S100A8 enriched in the immune and antivirus pathways were significantly changed when the hosts were infected with ASFV. The genes mentioned could play crucial roles in the process of the reaction to non-lethal ASF infection, which may will help to improve the ASF tolerance in the pig population through molecular breeding strategies. 2023-10-13 2023-10-26T14:42:13Z 2023-10-26T14:42:13Z Journal Article https://hdl.handle.net/10568/132485 en Open Access FapUNIFESP Feng, W., Zhou, L., Du, H., Okoth, E., Mrode, R., Jin, W., Hu, Z. and Liu, J.-F. 2023. Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus. Genetics and Molecular Biology 46(3): e20230037. |
| spellingShingle | animal diseases african swine fever swine genetics Feng, W. Zhou, L. Du, H. Okoth, Edward A. Mrode, Raphael A. Jin, W. Hu, Z. Liu, J.-F. Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus |
| title | Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus |
| title_full | Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus |
| title_fullStr | Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus |
| title_full_unstemmed | Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus |
| title_short | Transcriptome analysis reveals gene expression changes of pigs infected with non-lethal African swine fever virus |
| title_sort | transcriptome analysis reveals gene expression changes of pigs infected with non lethal african swine fever virus |
| topic | animal diseases african swine fever swine genetics |
| url | https://hdl.handle.net/10568/132485 |
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