Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed
Measurement of the nutritive value of feedstuffs with near infrared reflectance spectroscopy (NIRS) laboratory benchtop spectrometers is well-established. The aim of this study was to examine the reliability and accuracy of a handheld low-cost Tellspec NIRS spectrometer for measurement of the nutrit...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Frontiers Media
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/131194 |
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| author | Walelegne, M. Meheret, F. Bezabih, Melkamu Dejene, M. Asmare, Yonas Prasad, Kodukula V.S.V. Jones, Christopher S. Dixon, R.M. Duncan, Alan J. |
| author_browse | Asmare, Yonas Bezabih, Melkamu Dejene, M. Dixon, R.M. Duncan, Alan J. Jones, Christopher S. Meheret, F. Prasad, Kodukula V.S.V. Walelegne, M. |
| author_facet | Walelegne, M. Meheret, F. Bezabih, Melkamu Dejene, M. Asmare, Yonas Prasad, Kodukula V.S.V. Jones, Christopher S. Dixon, R.M. Duncan, Alan J. |
| author_sort | Walelegne, M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Measurement of the nutritive value of feedstuffs with near infrared reflectance spectroscopy (NIRS) laboratory benchtop spectrometers is well-established. The aim of this study was to examine the reliability and accuracy of a handheld low-cost Tellspec NIRS spectrometer for measurement of the nutritive value of oilseed meals.Samples (n=142) comprising byproduct meals from processing linseed, noug seed, cotton seed, groundnut, rapeseed, soybeans, and sunflower seeds were collected from farms, oil factories, wholesalers, and retail shops in central Ethiopia. Samples were scanned ‘as received’ (UGr) and also following drying and grinding (Gr), and were scanned once, twice, and ten times in a 2x3 factorial experimental design. Laboratory analyses of total nitrogen (TN), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and in vitro organic matter digestibility (IVOMD) provided reference measurements. Calibration models were developed using a subset of 2/3 of the spectra and validated using the remaining 1/3 of sample spectra.The sample form and the number of scans, and their interactions, all significantly affected the accuracy of the calibration models (P<0.001). The most accurate calibrations were with Gr samples scanned 10 times, where the coefficient of determination of both calibration and validation sets (R2cal and R2val) were ≥ 0.90 for most attributes. The respective standard errors of prediction (SEP) (g/kg DM) for Gr, and ‘as received’, samples respectively that were scanned ten times were: TN (3.2 and 4.7), IVOMD (11.7 and 20.1), NDF (26.9 and 43.8), ADF (25.3 and 44.6), and ADL (6.8 and 10.7). Also, the SEP for each attribute was reduced (P<0.05) by drying and grinding the sample before scanning. The ratios of the standard deviation of the calibration samples to the SEP (RPD) g/kg DM were 2.68, 2.30, 3.17, 3.05, and 4.06 for TN, IVOMD, NDF, ADF, and ADL, respectively, for Gr samples scanned ten times. Nevertheless, the SEP of samples scanned ‘as received’ would often be acceptable for routine analyses in the field and market-place under east African circumstances.In conclusion, NIRS calibrations for a portable handheld Tellspec NIRS spectrometer could be developed to measure important nutritional attributes of oilseed cake samples as feeds. |
| format | Journal Article |
| id | CGSpace131194 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace1311942025-12-08T10:29:22Z Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed Walelegne, M. Meheret, F. Bezabih, Melkamu Dejene, M. Asmare, Yonas Prasad, Kodukula V.S.V. Jones, Christopher S. Dixon, R.M. Duncan, Alan J. infrared spectrophotometry livestock feeds oilseeds animal feeding Measurement of the nutritive value of feedstuffs with near infrared reflectance spectroscopy (NIRS) laboratory benchtop spectrometers is well-established. The aim of this study was to examine the reliability and accuracy of a handheld low-cost Tellspec NIRS spectrometer for measurement of the nutritive value of oilseed meals.Samples (n=142) comprising byproduct meals from processing linseed, noug seed, cotton seed, groundnut, rapeseed, soybeans, and sunflower seeds were collected from farms, oil factories, wholesalers, and retail shops in central Ethiopia. Samples were scanned ‘as received’ (UGr) and also following drying and grinding (Gr), and were scanned once, twice, and ten times in a 2x3 factorial experimental design. Laboratory analyses of total nitrogen (TN), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and in vitro organic matter digestibility (IVOMD) provided reference measurements. Calibration models were developed using a subset of 2/3 of the spectra and validated using the remaining 1/3 of sample spectra.The sample form and the number of scans, and their interactions, all significantly affected the accuracy of the calibration models (P<0.001). The most accurate calibrations were with Gr samples scanned 10 times, where the coefficient of determination of both calibration and validation sets (R2cal and R2val) were ≥ 0.90 for most attributes. The respective standard errors of prediction (SEP) (g/kg DM) for Gr, and ‘as received’, samples respectively that were scanned ten times were: TN (3.2 and 4.7), IVOMD (11.7 and 20.1), NDF (26.9 and 43.8), ADF (25.3 and 44.6), and ADL (6.8 and 10.7). Also, the SEP for each attribute was reduced (P<0.05) by drying and grinding the sample before scanning. The ratios of the standard deviation of the calibration samples to the SEP (RPD) g/kg DM were 2.68, 2.30, 3.17, 3.05, and 4.06 for TN, IVOMD, NDF, ADF, and ADL, respectively, for Gr samples scanned ten times. Nevertheless, the SEP of samples scanned ‘as received’ would often be acceptable for routine analyses in the field and market-place under east African circumstances.In conclusion, NIRS calibrations for a portable handheld Tellspec NIRS spectrometer could be developed to measure important nutritional attributes of oilseed cake samples as feeds. 2023-07-07 2023-07-17T14:00:31Z 2023-07-17T14:00:31Z Journal Article https://hdl.handle.net/10568/131194 en Open Access Frontiers Media Walelegne, M., Meheret, F., Bezabih, M., Dejene, M., Asmare, Y., Prasad, K.V.S.V., Jones, C.S., Dixon, R.M. and Duncan, A.J. 2023. Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed. Frontiers in Animal Science 4:1203449. |
| spellingShingle | infrared spectrophotometry livestock feeds oilseeds animal feeding Walelegne, M. Meheret, F. Bezabih, Melkamu Dejene, M. Asmare, Yonas Prasad, Kodukula V.S.V. Jones, Christopher S. Dixon, R.M. Duncan, Alan J. Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| title | Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| title_full | Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| title_fullStr | Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| title_full_unstemmed | Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| title_short | Near-infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| title_sort | near infrared reflectance spectroscopy using a portable instrument to measure the nutritive value of oilseed meals as livestock feed |
| topic | infrared spectrophotometry livestock feeds oilseeds animal feeding |
| url | https://hdl.handle.net/10568/131194 |
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