Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU

Wheat is one of the most important crops for global food security and represents a main source of food and income for millions of smallholder farmers worldwide. Pseudomonas syringae pv. syringae (Pss) is the causal agent of bacterial leaf blight or leaf necrosis disease with specific symptoms on whe...

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Autores principales: Kumari, Safaa, Moukahel, Abdulrahman
Formato: Tool
Lenguaje:Inglés
Publicado: International Center for Agricultural Research in the Dry Areas 2022
Materias:
Acceso en línea:https://hdl.handle.net/10568/127948
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author Kumari, Safaa
Moukahel, Abdulrahman
author_browse Kumari, Safaa
Moukahel, Abdulrahman
author_facet Kumari, Safaa
Moukahel, Abdulrahman
author_sort Kumari, Safaa
collection Repository of Agricultural Research Outputs (CGSpace)
description Wheat is one of the most important crops for global food security and represents a main source of food and income for millions of smallholder farmers worldwide. Pseudomonas syringae pv. syringae (Pss) is the causal agent of bacterial leaf blight or leaf necrosis disease with specific symptoms on wheat leaves and spikes and in good conditions it can reach to the seeds to be seed borne disease. Yield losses caused by Pss can reach up to 50% or more depending on the crop, time of infection, and region. Therefore, developing and implementing effective management strategies for bacterial diseases is very important to reduce yield and quality loss. However, accurate detection of diseases is the first essential step for effective management strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the occurrence and development of crop diseases. This report describes a validated conventional polymerase chain reaction (PCR) assay for Pss detection and characterization applied by ICARDA’s GHU.
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spelling CGSpace1279482026-01-14T02:07:31Z Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU Kumari, Safaa Moukahel, Abdulrahman bacterial diseases plant protection plant health seed testing wheat seed-borne diseases food secuity molecular detection Wheat is one of the most important crops for global food security and represents a main source of food and income for millions of smallholder farmers worldwide. Pseudomonas syringae pv. syringae (Pss) is the causal agent of bacterial leaf blight or leaf necrosis disease with specific symptoms on wheat leaves and spikes and in good conditions it can reach to the seeds to be seed borne disease. Yield losses caused by Pss can reach up to 50% or more depending on the crop, time of infection, and region. Therefore, developing and implementing effective management strategies for bacterial diseases is very important to reduce yield and quality loss. However, accurate detection of diseases is the first essential step for effective management strategies for control of this disease. Molecular is efficient tool to accurately detect and monitor the occurrence and development of crop diseases. This report describes a validated conventional polymerase chain reaction (PCR) assay for Pss detection and characterization applied by ICARDA’s GHU. 2022-12-01 2023-01-24T03:08:27Z 2023-01-24T03:08:27Z Tool https://hdl.handle.net/10568/127948 en Open Access application/pdf International Center for Agricultural Research in the Dry Areas Safaa G. Kumari and Abdelrahman Moukahel. 2022. Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU. International Center for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon.
spellingShingle bacterial diseases
plant protection
plant health
seed testing
wheat
seed-borne diseases
food secuity
molecular detection
Kumari, Safaa
Moukahel, Abdulrahman
Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU
title Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU
title_full Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU
title_fullStr Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU
title_full_unstemmed Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU
title_short Conventional polymerase chain reaction (PCR) assay for detection and characterization of bacterial leaf blight in wheat applied by ICARDA’s GHU
title_sort conventional polymerase chain reaction pcr assay for detection and characterization of bacterial leaf blight in wheat applied by icarda s ghu
topic bacterial diseases
plant protection
plant health
seed testing
wheat
seed-borne diseases
food secuity
molecular detection
url https://hdl.handle.net/10568/127948
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